Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX105620 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX105620, RRID:AB_1949621
- Product name
- AKR1C1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Preferential expression of potential markers for cancer stem cells in large cell neuroendocrine carcinoma of the lung. An FFPE proteomic study.
Nomura M, Fukuda T, Fujii K, Kawamura T, Tojo H, Kihara M, Bando Y, Gazdar AF, Tsuboi M, Oshiro H, Nagao T, Ohira T, Ikeda N, Gotoh N, Kato H, Marko-Varga G, Nishimura T
Journal of clinical bioinformatics 2011 Sep 3;1(1):23
Journal of clinical bioinformatics 2011 Sep 3;1(1):23
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Supportive validation
- Submitted by
- GeneTex (provider)
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- Experimental details
- Sample(30 ?g whole cell lysate)A:Raji (GTX27908)10% SDS PAGEGTX105620 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- AKR1C1 antibody detects AKR1C1 protein by western blot analysis.A. 50 ?g mouse liver lysate/extract10% SDS-PAGEAKR1C1 antibody (GTX105620) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C1 antibody (GTX105620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE GTX105620 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed A431, using AKR1C1(GTX105620) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft , using AKR1C1(GTX105620) antibody at 1:500 dilution.