PA5-21672

antibody from Invitrogen Antibodies

Targeting: AKR1C1 DD1, DDH, DDH1, HAKRC, MBAB

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21672

Provider

Invitrogen Antibodies

Product name

AKR1C1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, HeLa, HepG2, A549, mouse liver. Predicted reactivity: Pig (83%), Rhesus Monkey (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.6 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Regulation of uterine function during estrous cycle, anestrus phase and pregnancy by steroids in red deer (Cervus elaphus L.).

Kotlarczyk AM, Grzyb M, Korzekwa AJ
Scientific reports 2021 Oct 11;11(1):20109

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of AKR1C1 in methanol-fixed A431 cells using an AKR1C1 polyclonal antibody (Product # PA5-21672) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed A431, using AKR1C1 antibody (Product # PA5-21672) at 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed A431, using AKR1C1 antibody (Product # PA5-21672) at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft , using AKR1C1 (Product # PA5-21672) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 mRNA and protein expression of AKR1C1 ( A , E ), P450 ( B , F ), 3beta-HSD ( C , G ) and 17beta-HSD ( D , H ) in uterine tissues (endometrium and myometrium) on 4th and 13th day of estrous cycle, in pregnancy and anestrus phase. Data were normalized against GAPDH for mRNA expression and against beta-actin (ACTB) for proteins expression. Each bar represents one experimental group with SEM. Statistical differences were analyzed by two-way analysis (ANOVA) of variance followed by the Bonferroni post hoc test using GraphPad PRISM (Version 8.3.0). The lowest statistical significance was P < 0.05. Asterisks indicate statistical differences between endometrium and myometrium (* P < 0.1; ** P < 0.01; *** P < 0.001; **** P < 0.0001). Different letters indicate statistical differences ( P < 0.05) between the experimental groups throughout endometrium (A, B) and myometrium (a-b) respectively.