PA5-97764

antibody from Invitrogen Antibodies

Targeting: G0S2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-97764

Provider

Invitrogen Antibodies

Product name

G0S2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

3 mg/mL

Storage

-20°C or -80°C if preferred

References

Submitted references

Lung mesenchymal cells elicit lipid storage in neutrophils that fuel breast cancer lung metastasis.

Li P, Lu M, Shi J, Gong Z, Hua L, Li Q, Lim B, Zhang XH, Chen X, Li S, Shultz LD, Ren G
Nature immunology 2020 Nov;21(11):1444-1455

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of G0S2 using a G0S2 Polyclonal antibody (Product # PA5-97764) at a concentration of 2 µg/mL. Positive WB detected in Mouse heart tissue. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:10,000 dilution. Observed band size: 20 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Extended Data Fig. 2 Up-regulation of genes encoding ATGL inhibitory factors led to reduced TG hydrolysis in pre-metastatic lung-infiltrating neutrophils a-h , Levels of total triglyceride (TG) ( a ), cholesteryl ester (CE) ( b ), phosphatidylethanolamine (PE) ( c ), phosphatidylcholine (PC) ( d ), phosphatidylserine (PS) ( e ), phosphatidylinositol (PI) ( f ), ceramides (Cer) ( g ) and phosphatidylglycerol (PG) ( h ) in PB and lung neutrophils isolated from the pre-metastatic stage of orthotopic 4T1 tumor-bearing mice (n = 5), were determined by liquid chromatography-mass spectrometry. Relative levels of these lipid were also shown in Fig. 1f . The exact values of specific chemical species for each lipid type were provided in Source Data file. i-l , Neutrophils were isolated from the BM, PB and lung tissues of orthotopic 4T1 tumor-bearing mice (n = 5) at the pre-metastatic stage and compared for relative mRNA expression ( to Rps18 ) of Hilpda , Cidec , G0s2 , Atgl and Abhd5 by qRT-PCR ( i ); HILPDA, CIDEC and G0S2 protein expression by Western blotting with GAPDH as a loading control ( j ); relative TG hydrolase activity ( k ); and cellular lipase activity ( l ). n represents biologically independent animals. Data are mean +- s.e.m. and P values were determined by one-way ANOVA with Tukey''s multiple comparisons test ( i and l ) or unpaired two-tailed t -test ( k ). ns, not significant.