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Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- MA5-24076 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Claudin 6 Monoclonal Antibody (342927)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody stains human Claudin-6 transfected cells but not irrelevant transfectants. Reconstitute at 0.5 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 342927
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Epicardium-derived cells organize through tight junctions to replenish cardiac muscle in salamanders.
Eroglu E, Yen CYT, Tsoi YL, Witman N, Elewa A, Joven Araus A, Wang H, Szattler T, Umeano CH, Sohlmér J, Goedel A, Simon A, Chien KR
Nature cell biology 2022 May;24(5):645-658
Nature cell biology 2022 May;24(5):645-658
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Claudin-6 was detected in immersion fixed iPS2 human induced pluripotent stem cells, differentiated into definitive endoderm with StemXVivo Endoderm Kit, using mouse Anti-human Claudin-6 Monoclonal Antibody (Product # MA5-24076) at 10 µg/mL for 3 hours at room temperature. Cells were stained using a 557-conjugated Anti-mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). SOX17 was also detected using Goat Anti-human SOX17 Antigen Affinity-purified Polyclonal Antibody (green). Specific staining of Claudin-6 was localized to cell surfaces.
