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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- MA5-37461 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Claudin 6 Monoclonal Antibody (AFB17981)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Antibody detects endogenous levels of total CLDN6.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- AFB17981
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Claudin 6 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Claudin 6 monoclonal antibody (Product # MA5-37461) and rabbit anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-mouse IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Claudin 6 in human liver cancer tissue sections. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with monoclonal antibody (Product # MA5-37461) at a dilution of 1:200. Secondary staining was applied with HRP conjugated goat anti-Mouse.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Claudin 6 in human lung cancer tissue sections. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with monoclonal antibody (Product # MA5-37461) at a dilution of 1:200. Secondary staining was applied with HRP conjugated goat anti-Mouse.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Claudin 6 in human breast cancer tissue sections. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with monoclonal antibody (Product # MA5-37461) at a dilution of 1:200. Secondary staining was applied with HRP conjugated goat anti-Mouse.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Claudin 6 in human liver cancer tissue sections. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with monoclonal antibody (Product # MA5-37461) at a dilution of 1:200. Secondary staining was applied with HRP conjugated goat anti-Mouse.
