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- Antibody Data
- Antigen structure
- References [18]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- 66191-1-Ig - Provider product page
- Provider
- Proteintech Group
- Product name
- VAPB antibody
- Antibody type
- Monoclonal
- Description
- KD/KO validated VAPB antibody (Cat. #66191-1-Ig) is a mouse monoclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, WB, ELISA.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 4F6A6
- Vial size
- 20ul, 150ul
Submitted references Protocol for measuring interorganelle contact sites in primary cells using a modified proximity ligation assay.
Characterization of mitochondria-associated ER membranes in the enteric nervous system under physiological and pathological conditions.
Mitochondria-associated endoplasmic reticulum membranes tethering protein VAPB-PTPIP51 protects against ischemic stroke through inhibiting the activation of autophagy.
Progression of herpesvirus infection remodels mitochondrial organization and metabolism.
Super-Resolution Imaging of Peroxisomal Proteins Using STED Nanoscopy.
Assessing Peroxisomal Protein Interaction by Immunoprecipitation.
Proximity-Ligation Assay to Detect Peroxisome-Organelle Interaction.
A SNX1-SNX2-VAPB partnership regulates endosomal membrane rewiring in response to nutritional stress.
An obligate intracellular bacterial pathogen forms a direct, interkingdom membrane contact site.
Calcium transients on the ER surface trigger liquid-liquid phase separation of FIP200 to specify autophagosome initiation sites.
MITOL regulates phosphatidic acid-binding activity of RMDN3/PTPIP51.
Restructured membrane contacts rewire organelles for human cytomegalovirus infection.
Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy.
CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy.
Proteomic mapping by rapamycin-dependent targeting of APEX2 identifies binding partners of VAPB at the inner nuclear membrane.
Fluorescent tools to analyse peroxisome-ER interactions in mammalian cells.
Host Vesicle Fusion Protein VAPB Contributes to the Nuclear Egress Stage of Herpes Simplex Virus Type-1 (HSV-1) Replication.
The ER Contact Proteins VAPA/B Interact with Multiple Autophagy Proteins to Modulate Autophagosome Biogenesis.
Ilamathi HS, Benhammouda S, Chatel-Chaix L, Germain M
STAR protocols 2024 Mar 15;5(1):102915
STAR protocols 2024 Mar 15;5(1):102915
Characterization of mitochondria-associated ER membranes in the enteric nervous system under physiological and pathological conditions.
Delfino G, Briand JB, Oullier T, Nienkemper L, Greig J, Véziers J, Neunlist M, Derkinderen P, Paillusson S
American journal of physiology. Gastrointestinal and liver physiology 2024 Mar 1;326(3):G330-G343
American journal of physiology. Gastrointestinal and liver physiology 2024 Mar 1;326(3):G330-G343
Mitochondria-associated endoplasmic reticulum membranes tethering protein VAPB-PTPIP51 protects against ischemic stroke through inhibiting the activation of autophagy.
Li M, Zhang Y, Yu G, Gu L, Zhu H, Feng S, Xiong X, Jian Z
CNS neuroscience & therapeutics 2024 Apr;30(4):e14707
CNS neuroscience & therapeutics 2024 Apr;30(4):e14707
Progression of herpesvirus infection remodels mitochondrial organization and metabolism.
Leclerc S, Gupta A, Ruokolainen V, Chen JH, Kunnas K, Ekman AA, Niskanen H, Belevich I, Vihinen H, Turkki P, Perez-Berna AJ, Kapishnikov S, Mäntylä E, Harkiolaki M, Dufour E, Hytönen V, Pereiro E, McEnroe T, Fahy K, Kaikkonen MU, Jokitalo E, Larabell CA, Weinhardt V, Mattola S, Aho V, Vihinen-Ranta M
PLoS pathogens 2024 Apr;20(4):e1011829
PLoS pathogens 2024 Apr;20(4):e1011829
Super-Resolution Imaging of Peroxisomal Proteins Using STED Nanoscopy.
de Lange EMF, Vlijm R
Methods in molecular biology (Clifton, N.J.) 2023;2643:65-84
Methods in molecular biology (Clifton, N.J.) 2023;2643:65-84
Assessing Peroxisomal Protein Interaction by Immunoprecipitation.
Kors S, Schrader M
Methods in molecular biology (Clifton, N.J.) 2023;2643:345-357
Methods in molecular biology (Clifton, N.J.) 2023;2643:345-357
Proximity-Ligation Assay to Detect Peroxisome-Organelle Interaction.
Kamoshita M, Schrader M
Methods in molecular biology (Clifton, N.J.) 2023;2643:135-148
Methods in molecular biology (Clifton, N.J.) 2023;2643:135-148
A SNX1-SNX2-VAPB partnership regulates endosomal membrane rewiring in response to nutritional stress.
Da Graça J, Charles J, Djebar M, Alvarez-Valadez K, Botti J, Morel E
Life science alliance 2023 Mar;6(3)
Life science alliance 2023 Mar;6(3)
An obligate intracellular bacterial pathogen forms a direct, interkingdom membrane contact site.
Acevedo-Sánchez Y, Woida PJ, Kraemer S, Lamason RL
bioRxiv : the preprint server for biology 2023 Jun 6;
bioRxiv : the preprint server for biology 2023 Jun 6;
Calcium transients on the ER surface trigger liquid-liquid phase separation of FIP200 to specify autophagosome initiation sites.
Zheng Q, Chen Y, Chen D, Zhao H, Feng Y, Meng Q, Zhao Y, Zhang H
Cell 2022 Oct 27;185(22):4082-4098.e22
Cell 2022 Oct 27;185(22):4082-4098.e22
MITOL regulates phosphatidic acid-binding activity of RMDN3/PTPIP51.
Ito N, Takahashi T, Shiiba I, Nagashima S, Inatome R, Yanagi S
Journal of biochemistry 2022 May 11;171(5):529-541
Journal of biochemistry 2022 May 11;171(5):529-541
Restructured membrane contacts rewire organelles for human cytomegalovirus infection.
Cook KC, Tsopurashvili E, Needham JM, Thompson SR, Cristea IM
Nature communications 2022 Aug 11;13(1):4720
Nature communications 2022 Aug 11;13(1):4720
Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy.
Liu N, Zhao H, Zhao YG, Hu J, Zhang H
The Journal of cell biology 2021 Jul 5;220(7)
The Journal of cell biology 2021 Jul 5;220(7)
CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy.
Nthiga TM, Kumar Shrestha B, Sjøttem E, Bruun JA, Bowitz Larsen K, Bhujabal Z, Lamark T, Johansen T
The EMBO journal 2020 Aug 3;39(15):e103649
The EMBO journal 2020 Aug 3;39(15):e103649
Proteomic mapping by rapamycin-dependent targeting of APEX2 identifies binding partners of VAPB at the inner nuclear membrane.
James C, Müller M, Goldberg MW, Lenz C, Urlaub H, Kehlenbach RH
The Journal of biological chemistry 2019 Nov 1;294(44):16241-16254
The Journal of biological chemistry 2019 Nov 1;294(44):16241-16254
Fluorescent tools to analyse peroxisome-ER interactions in mammalian cells.
Bishop A, Kamoshita M, Passmore JB, Hacker C, Schrader TA, Waterham HR, Costello JL, Schrader M
Contact (Thousand Oaks (Ventura County, Calif.)) 2019 Jun 5;2
Contact (Thousand Oaks (Ventura County, Calif.)) 2019 Jun 5;2
Host Vesicle Fusion Protein VAPB Contributes to the Nuclear Egress Stage of Herpes Simplex Virus Type-1 (HSV-1) Replication.
Saiz-Ros N, Czapiewski R, Epifano I, Stevenson A, Swanson SK, Dixon CR, Zamora DB, McElwee M, Vijayakrishnan S, Richardson CA, Dong L, Kelly DA, Pytowski L, Goldberg MW, Florens L, Graham SV, Schirmer EC
Cells 2019 Feb 3;8(2)
Cells 2019 Feb 3;8(2)
The ER Contact Proteins VAPA/B Interact with Multiple Autophagy Proteins to Modulate Autophagosome Biogenesis.
Zhao YG, Liu N, Miao G, Chen Y, Zhao H, Zhang H
Current biology : CB 2018 Apr 23;28(8):1234-1245.e4
Current biology : CB 2018 Apr 23;28(8):1234-1245.e4
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 66191-1-Ig( VAPB Antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The VAPB antibody from Proteintech is a mouse monoclonal antibody to a recombinant protein of human VAPB. This antibody recognizes human antigen. The VAPB antibody has been validated for the following applications: ELISA, WB, IF, IHC analysis.
