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- Antibody Data
- Antigen structure
- References [72]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- 14477-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14477-1-AP, RRID:AB_2288297
- Product name
- VAPB antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated VAPB antibody (Cat. #14477-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Fasting Enhances Cardiomyocyte Hypoxia Tolerance by Regulating Ca(2+) Transport at Mitochondria-Endoplasmic Reticulum Contact Sites.
Pathogenic mechanisms of amyotrophic lateral sclerosis-linked VAPB P56S mutation in the degeneration of corticospinal motor neurons.
Serine-129 phosphorylated α-synuclein drives mitochondrial dysfunction and calcium dysregulation in Parkinson's disease model.
Convergent activation of the integrated stress response and ER-mitochondria uncoupling in VAPB-associated ALS.
Neurons and astrocytes have distinct organelle signatures and responses to stress.
Electroacupuncture Regulates Mitochondria-Endoplasmic Reticulum interactions via Fn1 in a Parkinson's Disease Model: Transcriptomic Evidence.
Protein Kinase C promotes peroxisome biogenesis and peroxisome-endoplasmic reticulum interaction.
Peri-mitochondrial actin filaments inhibit Parkin assembly by disrupting ER-mitochondria contacts.
Elevated synaptic PKA activity and abnormal striatal dopamine signaling in Akap11 mutant mice, a genetic model of schizophrenia and bipolar disorder.
Bipolar and schizophrenia risk gene AKAP11 encodes an autophagy receptor coupling the regulation of PKA kinase network homeostasis to synaptic transmission.
Sec14L6 is a phosphoinositide transporter that regulates phosphoinositide homeostasis and biogenesis of lipid droplets.
Elevated synaptic PKA activity and abnormal striatal dopamine signaling in Akap11 mutant mice, a genetic model of schizophrenia and bipolar disorder.
FAM134B-mediated ER-phagy alleviates alcohol-related liver fibrosis by reducing endoplasmic reticulum stress.
Rickettsia parkeri forms extensive, stable contacts with the rough endoplasmic reticulum.
Bipolar and schizophrenia risk gene AKAP11 encodes an autophagy receptor coupling the regulation of PKA kinase network homeostasis to synaptic transmission.
Fluoride induces spermatocyte apoptosis by IP3R1/MCU-mediated mitochondrial calcium overload through MAMs.
ER-endosome contacts generate a local environment promoting phagophore formation.
Tail Anchored protein insertion mediated by CAML and TRC40 links to neuromuscular function in mice.
Elevating VAPB-PTPIP51 integration repairs damaged mitochondria-associated endoplasmic reticulum membranes and inhibits lung fibroblasts activation.
Bridging the gap: investigating the role of phosphorylation at the serine 129 site of α-synuclein in VAPB-PTPIP51 interactions.
Changes in the endoplasmic reticulum‑mitochondria communication in dermal fibroblasts from early‑stage bipolar disorder patients: Skin‑brain axis as a new route to understand the pathophysiology of mental illness?
Toxoplasma effector TgROP1 establishes membrane contact sites with the endoplasmic reticulum during infection.
VAPB is a negative regulator of STING-mediated innate immune signaling.
Ribosome-binding protein 1 maintains peroxisome biogenesis.
RHOA regulates mitochondria-ER contact sites through modulation of the VAPB/PTPIP51 tether.
The p97-UBXD8 complex maintains peroxisome abundance by suppressing pexophagy.
Development of a Signal-integrating Reporter to Monitor Mitochondria-ER Contacts.
GRP75-dependent mitochondria-ER contacts ensure cell survival during early mouse thymocyte development.
Fluorescent fatty acid conjugates for live cell imaging of peroxisomes.
Mitochondrial dysfunction heightens the integrated stress response to drive ALS pathogenesis.
CGI1746 targets σ(1)R to modulate ferroptosis through mitochondria-associated membranes.
ARTC1-mediated VAPB ADP-ribosylation regulates calcium homeostasis.
VAP spatially stabilizes dendritic mitochondria to locally support synaptic plasticity.
Development of a signal-integrating reporter to monitor mitochondria-ER contacts.
Particulate matter induced cognitive impairments via endoplasmic reticulum stress-mediated damage to mitochondria-associated endoplasmic reticulum membranes in immature rats.
A novel bacterial effector protein mediates ER-LD membrane contacts to regulate host lipid droplets.
USP20 deubiquitinates and stabilizes the reticulophagy receptor RETREG1/FAM134B to drive reticulophagy.
Phenylalanine deprivation inhibits multiple myeloma progression by perturbing endoplasmic reticulum homeostasis.
ALS-Linked VapB P56S Mutation Alters Neuronal Mitochondrial Turnover at the Synapse.
Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats.
A SNX1-SNX2-VAPB partnership regulates endosomal membrane rewiring in response to nutritional stress.
Deficiency of Perry syndrome-associated p150(Glued) in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities.
Elucidation of OSW-1-Induced Stress Responses in Neuro2a Cells.
Oligomeric CHMP7 mediates three-way ER junctions and ER-mitochondria interactions.
The p97-UBXD8 complex regulates ER-Mitochondria contact sites by altering membrane lipid saturation and composition.
The novel bacterial effector protein CbEPF1 mediates ER-LD membrane contacts to regulate host lipid droplet metabolism.
ER-mitochondria contacts and cholesterol metabolism are disrupted by disease-associated tau protein.
Deregulation of ER-mitochondria contact formation and mitochondrial calcium homeostasis mediated by VDAC in fragile X syndrome.
SPTLC1 variants associated with ALS produce distinct sphingolipid signatures through impaired interaction with ORMDL proteins.
Proteomic Mapping and Targeting of Mitotic Pericentriolar Material in Tumors Bearing Centrosome Amplification.
Serine palmitoyltransferase assembles at ER-mitochondria contact sites.
Mobile late endosomes modulate peripheral endoplasmic reticulum network architecture.
The role of Mfn2 in the structure and function of endoplasmic reticulum-mitochondrial tethering in vivo.
The Novel ALG-2 Target Protein CDIP1 Promotes Cell Death by Interacting with ESCRT-I and VAPA/B.
MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.
VPS13D interacts with VCP/p97 and negatively regulates endoplasmic reticulum-mitochondria interactions.
A High-Density Human Mitochondrial Proximity Interaction Network.
Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation.
Nir2 Is an Effector of VAPs Necessary for Efficient Hepatitis C Virus Replication and Phosphatidylinositol 4-Phosphate Enrichment at the Viral Replication Organelle.
A trap mutant reveals the physiological client spectrum of TRC40.
MIGA2 Links Mitochondria, the ER, and Lipid Droplets and Promotes De Novo Lipogenesis in Adipocytes.
Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways.
Loss of VAPB Regulates Autophagy in a Beclin 1-Dependent Manner.
DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.
Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR.
Adenovirus Modulates Toll-Like Receptor 4 Signaling by Reprogramming ORP1L-VAP Protein Contacts for Cholesterol Transport from Endosomes to the Endoplasmic Reticulum.
Neuronal overexpression of human VAPB slows motor impairment and neuromuscular denervation in a mouse model of ALS.
The ATF6 pathway of the ER stress response contributes to enhanced viability in glioblastoma.
The role of endoplasmic reticulum-related BiP/GRP78 in interferon gamma-induced persistent Chlamydia pneumoniae infection.
Expression of vesicle-associated membrane-protein-associated protein B cleavage products in peripheral blood leukocytes and cerebrospinal fluid of patients with sporadic amyotrophic lateral sclerosis.
Widespread aggregation of mutant VAPB associated with ALS does not cause motor neuron degeneration or modulate mutant SOD1 aggregation and toxicity in mice.
Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation.
Chen X, Jiao B, Xue T, Xie M, Yu Z
International journal of molecular sciences 2026 Feb 24;27(5)
International journal of molecular sciences 2026 Feb 24;27(5)
Pathogenic mechanisms of amyotrophic lateral sclerosis-linked VAPB P56S mutation in the degeneration of corticospinal motor neurons.
Yang X, Zheng J, Wang X, Cai H, Yu J
Ageing and neurodegenerative diseases 2025;5(3)
Ageing and neurodegenerative diseases 2025;5(3)
Serine-129 phosphorylated α-synuclein drives mitochondrial dysfunction and calcium dysregulation in Parkinson's disease model.
Jiao J, Liu W, Gao G, Yang H
Frontiers in aging neuroscience 2025;17:1538166
Frontiers in aging neuroscience 2025;17:1538166
Convergent activation of the integrated stress response and ER-mitochondria uncoupling in VAPB-associated ALS.
Landry C, Costanzo JP, Mitne-Neto M, Zatz M, Schaffer AE, Hatzoglou M, Muotri AR, Miranda HC
EMBO molecular medicine 2025 Sep;17(9):2299-2331
EMBO molecular medicine 2025 Sep;17(9):2299-2331
Neurons and astrocytes have distinct organelle signatures and responses to stress.
Rhoads SN, Dong W, Hsu CH, Mfulama NR, Yarlagadda R, Ragusa JV, Ye M, Henrie A, Zanellati MC, Diering GH, Cohen TJ, Cohen S
Cell reports 2025 Sep 23;44(9):116280
Cell reports 2025 Sep 23;44(9):116280
Electroacupuncture Regulates Mitochondria-Endoplasmic Reticulum interactions via Fn1 in a Parkinson's Disease Model: Transcriptomic Evidence.
Lyu P, Wen F, Fu Z, Li J, Li Q, Cai X, Huang S, Qiu X, Zhang Z, Huang Y, Zhang J
Journal of integrative neuroscience 2025 Sep 18;24(9):40105
Journal of integrative neuroscience 2025 Sep 18;24(9):40105
Protein Kinase C promotes peroxisome biogenesis and peroxisome-endoplasmic reticulum interaction.
Borisyuk A, Howman C, Pattabiraman S, Kaganovich D, Amen T
The Journal of cell biology 2025 Sep 1;224(9)
The Journal of cell biology 2025 Sep 1;224(9)
Peri-mitochondrial actin filaments inhibit Parkin assembly by disrupting ER-mitochondria contacts.
Fung TS, Ghosh A, Zavala MR, Nichtova Z, Shukal D, Tigano M, Csordas G, Higgs HN, Chakrabarti R
EMBO reports 2025 Oct;26(20):4977-5008
EMBO reports 2025 Oct;26(20):4977-5008
Elevated synaptic PKA activity and abnormal striatal dopamine signaling in Akap11 mutant mice, a genetic model of schizophrenia and bipolar disorder.
Song BJ, Ge Y, Nicolella A, Kwon MJ, Lodder B, Bonanno K, Valle-Tojeiro A, Hartley ND, Perzel Mandell K, Adeleye J, Misri D, Geng C, Natarajan S, Picard I, Shepard N, Hall A, Tian J, Aryal S, Farsi Z, Liu XM, Morshed N, Nadaf NM, Pribiag H, Simmons SK, Mani DR, Stevens B, Kunwar PS, Fu Z, Macosko EZ, Levin JZ, Sabatini BL, Carr SA, Dejanovic B, Keshishian H, Granger AJ, Sheng M
Nature communications 2025 Nov 28;16(1):10793
Nature communications 2025 Nov 28;16(1):10793
Bipolar and schizophrenia risk gene AKAP11 encodes an autophagy receptor coupling the regulation of PKA kinase network homeostasis to synaptic transmission.
Lee YK, Xiao C, Zhou X, Wang L, McReynolds M, Wu Z, Han X, Purisic E, Kim H, Li X, Pang Z, Dai J, Peng J, Yang N, Yue Z
Nature communications 2025 Nov 28;16(1):10792
Nature communications 2025 Nov 28;16(1):10792
Sec14L6 is a phosphoinositide transporter that regulates phosphoinositide homeostasis and biogenesis of lipid droplets.
Zhou T, Xiong J, Hu X, Du Y, Shi A, Chang W, Qin J, Deng L, Ji WK
Nature communications 2025 Nov 26;16(1):10518
Nature communications 2025 Nov 26;16(1):10518
Elevated synaptic PKA activity and abnormal striatal dopamine signaling in Akap11 mutant mice, a genetic model of schizophrenia and bipolar disorder.
Song BJ, Ge Y, Nicolella A, Kwon MJ, Lodder B, Bonanno K, Valle-Tojeiro A, Hartley ND, Mandell KP, Adeleye J, Misri D, Geng C, Natarajan S, Picard I, Shepard N, Hall A, Tian J, Aryal S, Farsi Z, Liu XM, Morshed N, Nadaf NM, Pribiag H, Simmons SK, Mani DR, Stevens B, Kunwar PS, Fu Z, Macosko EZ, Levin JZ, Sabatini BL, Carr SA, Dejanovic B, Keshishian H, Granger AJ, Sheng M
bioRxiv : the preprint server for biology 2025 Nov 22;
bioRxiv : the preprint server for biology 2025 Nov 22;
FAM134B-mediated ER-phagy alleviates alcohol-related liver fibrosis by reducing endoplasmic reticulum stress.
Wang T, Li X, Xia G, Gong M, Lv X
International journal of biological macromolecules 2025 May;308(Pt 2):142395
International journal of biological macromolecules 2025 May;308(Pt 2):142395
Rickettsia parkeri forms extensive, stable contacts with the rough endoplasmic reticulum.
Acevedo-Sánchez Y, Woida PJ, Anderson C, Kraemer S, Lamason RL
The Journal of cell biology 2025 Mar 3;224(3)
The Journal of cell biology 2025 Mar 3;224(3)
Bipolar and schizophrenia risk gene AKAP11 encodes an autophagy receptor coupling the regulation of PKA kinase network homeostasis to synaptic transmission.
Lee YK, Xiao C, Zhou X, Wang L, McReynolds MG, Wu Z, Purisic E, Kim H, Li X, Pang Z, Dai J, Peng J, Yang N, Yue Z
Research square 2025 Mar 13;
Research square 2025 Mar 13;
Fluoride induces spermatocyte apoptosis by IP3R1/MCU-mediated mitochondrial calcium overload through MAMs.
Guo X, Wang L, Xuan J, Chen T, Du Y, Qiao H, Zhang S, Sun Z, Wang J, Niu R
Journal of hazardous materials 2025 Jun 5;489:137514
Journal of hazardous materials 2025 Jun 5;489:137514
ER-endosome contacts generate a local environment promoting phagophore formation.
Da Graça J, Thiola C, Rouabah M, Guerrera IC, El Khallouki N, Romao M, Alemany C, Amiri D, Dubois S, Srimoorthy A, Giordano F, Raposo G, Morel E
Cell reports 2025 Jul 22;44(7):115993
Cell reports 2025 Jul 22;44(7):115993
Tail Anchored protein insertion mediated by CAML and TRC40 links to neuromuscular function in mice.
Zhang Y, He L, Gundelach J, Ge A, Edlund H, Norlin S, Bram RJ
PLoS genetics 2025 Jan;21(1):e1011547
PLoS genetics 2025 Jan;21(1):e1011547
Elevating VAPB-PTPIP51 integration repairs damaged mitochondria-associated endoplasmic reticulum membranes and inhibits lung fibroblasts activation.
Ban J, Tian H, Wei Y, Ao L, Gu H, Zeng J, Peng X, Ao C, Zhang Y, He X, Zhao H, Li J
International immunopharmacology 2025 Feb 6;147:113982
International immunopharmacology 2025 Feb 6;147:113982
Bridging the gap: investigating the role of phosphorylation at the serine 129 site of α-synuclein in VAPB-PTPIP51 interactions.
Liu W, Lu Y, Liu J, Yu Y, Yang H
Acta neuropathologica communications 2025 Feb 24;13(1):40
Acta neuropathologica communications 2025 Feb 24;13(1):40
Changes in the endoplasmic reticulum‑mitochondria communication in dermal fibroblasts from early‑stage bipolar disorder patients: Skin‑brain axis as a new route to understand the pathophysiology of mental illness?
Pereira AC, Marques AP, Resende R, Serrano-Cuñarro L, Caldeira M, Fernandes T, Batista M, Macedo A, De Melo JB, Madeira N, Cavadas C, Cruz MT, Pereira CF
International journal of molecular medicine 2025 Dec;56(6)
International journal of molecular medicine 2025 Dec;56(6)
Toxoplasma effector TgROP1 establishes membrane contact sites with the endoplasmic reticulum during infection.
Mehra C, Alvarado Valverde J, Matias AMN, Torelli F, Medeiros TC, Straub J, Asaki JD, Bradley PJ, Luck K, Lawo S, Treeck M, Pernas L
Nature microbiology 2025 Dec;10(12):3331-3345
Nature microbiology 2025 Dec;10(12):3331-3345
VAPB is a negative regulator of STING-mediated innate immune signaling.
Ji W, Zhang Y, Zhang L, Liu Q, Niu S, Feng Y, Chen F, Liu X, Li X
Science advances 2025 Dec 5;11(49):eaea3996
Science advances 2025 Dec 5;11(49):eaea3996
Ribosome-binding protein 1 maintains peroxisome biogenesis.
Fatima K, Vihinen H, Akpinar A, Somborac T, Paatero A, Jokitalo E, Paavilainen V, Katajisto P, Konovalova S
Journal of cell science 2025 Dec 15;138(24)
Journal of cell science 2025 Dec 15;138(24)
RHOA regulates mitochondria-ER contact sites through modulation of the VAPB/PTPIP51 tether.
Yang Z, Nakajima S, Chen H, Chakrabarty Y, Feng H, Chan DC
Nature communications 2025 Dec 14;16(1):11260
Nature communications 2025 Dec 14;16(1):11260
The p97-UBXD8 complex maintains peroxisome abundance by suppressing pexophagy.
Montes ID, Amirthagunanathan S, Joshi AS, Raman M
bioRxiv : the preprint server for biology 2024 Sep 26;
bioRxiv : the preprint server for biology 2024 Sep 26;
Development of a Signal-integrating Reporter to Monitor Mitochondria-ER Contacts.
Yang Z, Chan DC
ACS synthetic biology 2024 Sep 20;13(9):2791-2803
ACS synthetic biology 2024 Sep 20;13(9):2791-2803
GRP75-dependent mitochondria-ER contacts ensure cell survival during early mouse thymocyte development.
Zhao F, Cui Z, Wang P, Zhao Z, Zhu K, Bai Y, Jin X, Wang L, Lu L
Developmental cell 2024 Oct 7;59(19):2643-2658.e7
Developmental cell 2024 Oct 7;59(19):2643-2658.e7
Fluorescent fatty acid conjugates for live cell imaging of peroxisomes.
Korotkova D, Borisyuk A, Guihur A, Bardyn M, Kuttler F, Reymond L, Schuhmacher M, Amen T
Nature communications 2024 May 21;15(1):4314
Nature communications 2024 May 21;15(1):4314
Mitochondrial dysfunction heightens the integrated stress response to drive ALS pathogenesis.
Landry C, Costanzo J, Mitne-Neto M, Zatz M, Schaffer A, Hatzoglou M, Muotri A, Miranda HC
bioRxiv : the preprint server for biology 2024 May 14;
bioRxiv : the preprint server for biology 2024 May 14;
CGI1746 targets σ(1)R to modulate ferroptosis through mitochondria-associated membranes.
Zhang Z, Zhou H, Gu W, Wei Y, Mou S, Wang Y, Zhang J, Zhong Q
Nature chemical biology 2024 Jun;20(6):699-709
Nature chemical biology 2024 Jun;20(6):699-709
ARTC1-mediated VAPB ADP-ribosylation regulates calcium homeostasis.
Ma X, Li M, Liu Y, Zhang X, Yang X, Wang Y, Li Y, Wang J, Liu X, Yan Z, Yu X, Wu C
Journal of molecular cell biology 2024 Jan 5;15(7)
Journal of molecular cell biology 2024 Jan 5;15(7)
VAP spatially stabilizes dendritic mitochondria to locally support synaptic plasticity.
Bapat O, Purimetla T, Kruessel S, Shah M, Fan R, Thum C, Rupprecht F, Langer JD, Rangaraju V
Nature communications 2024 Jan 4;15(1):205
Nature communications 2024 Jan 4;15(1):205
Development of a signal-integrating reporter to monitor mitochondria-ER contacts.
Yang Z, Chan DC
bioRxiv : the preprint server for biology 2024 Feb 14;
bioRxiv : the preprint server for biology 2024 Feb 14;
Particulate matter induced cognitive impairments via endoplasmic reticulum stress-mediated damage to mitochondria-associated endoplasmic reticulum membranes in immature rats.
Wang L, Gui J, Tian B, Ding R, Wang W, Jiang C, Zhang S, Zhang X, Liu J, Jiang L
Toxicology 2024 Dec;509:153979
Toxicology 2024 Dec;509:153979
A novel bacterial effector protein mediates ER-LD membrane contacts to regulate host lipid droplets.
Angara RK, Sadi A, Gilk SD
EMBO reports 2024 Dec;25(12):5331-5351
EMBO reports 2024 Dec;25(12):5331-5351
USP20 deubiquitinates and stabilizes the reticulophagy receptor RETREG1/FAM134B to drive reticulophagy.
Zhang M, Wang Z, Zhao Q, Yang Q, Bai J, Yang C, Zhang ZR, Liu Y
Autophagy 2024 Aug;20(8):1780-1797
Autophagy 2024 Aug;20(8):1780-1797
Phenylalanine deprivation inhibits multiple myeloma progression by perturbing endoplasmic reticulum homeostasis.
Cheng L, Wang X, Liu A, Zhu Y, Cheng H, Yu J, Gong L, Liu H, Shen G, Liu L
Acta pharmaceutica Sinica. B 2024 Aug;14(8):3493-3512
Acta pharmaceutica Sinica. B 2024 Aug;14(8):3493-3512
ALS-Linked VapB P56S Mutation Alters Neuronal Mitochondrial Turnover at the Synapse.
Wong HC, Lang AE, Stein C, Drerup CM
The Journal of neuroscience : the official journal of the Society for Neuroscience 2024 Aug 28;44(35)
The Journal of neuroscience : the official journal of the Society for Neuroscience 2024 Aug 28;44(35)
Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats.
Wang Y, Miao Z, Xu C, Cai Y, Yang Y, Hu Y, Zhao M, Shao Y, Li Z, Chen J, Chen S, Wang L
Cell death & disease 2023 May 13;14(5):325
Cell death & disease 2023 May 13;14(5):325
A SNX1-SNX2-VAPB partnership regulates endosomal membrane rewiring in response to nutritional stress.
Da Graça J, Charles J, Djebar M, Alvarez-Valadez K, Botti J, Morel E
Life science alliance 2023 Mar;6(3)
Life science alliance 2023 Mar;6(3)
Deficiency of Perry syndrome-associated p150(Glued) in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities.
Yu J, Yang X, Zheng J, Sgobio C, Sun L, Cai H
NPJ Parkinson's disease 2023 Mar 7;9(1):35
NPJ Parkinson's disease 2023 Mar 7;9(1):35
Elucidation of OSW-1-Induced Stress Responses in Neuro2a Cells.
Oh-Hashi K, Nakamura H, Ogawa H, Hirata Y, Sakurai K
International journal of molecular sciences 2023 Mar 17;24(6)
International journal of molecular sciences 2023 Mar 17;24(6)
Oligomeric CHMP7 mediates three-way ER junctions and ER-mitochondria interactions.
Chu Q, Wang J, Du Y, Zhou T, Shi A, Xiong J, Ji WK, Deng L
Cell death and differentiation 2023 Jan;30(1):94-110
Cell death and differentiation 2023 Jan;30(1):94-110
The p97-UBXD8 complex regulates ER-Mitochondria contact sites by altering membrane lipid saturation and composition.
Ganji R, Paulo JA, Xi Y, Kline I, Zhu J, Clemen CS, Weihl CC, Purdy JG, Gygi SP, Raman M
Nature communications 2023 Feb 6;14(1):638
Nature communications 2023 Feb 6;14(1):638
The novel bacterial effector protein CbEPF1 mediates ER-LD membrane contacts to regulate host lipid droplet metabolism.
Angara RK, Sadi A, Gilk SD
bioRxiv : the preprint server for biology 2023 Dec 11;
bioRxiv : the preprint server for biology 2023 Dec 11;
ER-mitochondria contacts and cholesterol metabolism are disrupted by disease-associated tau protein.
Szabo L, Cummins N, Paganetti P, Odermatt A, Papassotiropoulos A, Karch C, Götz J, Eckert A, Grimm A
EMBO reports 2023 Aug 3;24(8):e57499
EMBO reports 2023 Aug 3;24(8):e57499
Deregulation of ER-mitochondria contact formation and mitochondrial calcium homeostasis mediated by VDAC in fragile X syndrome.
Geng J, Khaket TP, Pan J, Li W, Zhang Y, Ping Y, Cobos Sillero MI, Lu B
Developmental cell 2023 Apr 10;58(7):597-615.e10
Developmental cell 2023 Apr 10;58(7):597-615.e10
SPTLC1 variants associated with ALS produce distinct sphingolipid signatures through impaired interaction with ORMDL proteins.
Lone MA, Aaltonen MJ, Zidell A, Pedro HF, Morales Saute JA, Mathew S, Mohassel P, Bönnemann CG, Shoubridge EA, Hornemann T
The Journal of clinical investigation 2022 Sep 15;132(18)
The Journal of clinical investigation 2022 Sep 15;132(18)
Proteomic Mapping and Targeting of Mitotic Pericentriolar Material in Tumors Bearing Centrosome Amplification.
Xie B, Pu Y, Yang F, Chen W, Yue W, Ma J, Zhang N, Jiang Y, Wu J, Lin Y, Liang X, Wang C, Zou P, Li M
Cancer research 2022 Jul 18;82(14):2576-2592
Cancer research 2022 Jul 18;82(14):2576-2592
Serine palmitoyltransferase assembles at ER-mitochondria contact sites.
Aaltonen MJ, Alecu I, König T, Bennett SA, Shoubridge EA
Life science alliance 2022 Feb;5(2)
Life science alliance 2022 Feb;5(2)
Mobile late endosomes modulate peripheral endoplasmic reticulum network architecture.
Spits M, Heesterbeek IT, Voortman LM, Akkermans JJ, Wijdeven RH, Cabukusta B, Neefjes J
EMBO reports 2021 Mar 3;22(3):e50815
EMBO reports 2021 Mar 3;22(3):e50815
The role of Mfn2 in the structure and function of endoplasmic reticulum-mitochondrial tethering in vivo.
Han S, Zhao F, Hsia J, Ma X, Liu Y, Torres S, Fujioka H, Zhu X
Journal of cell science 2021 Jul 1;134(13)
Journal of cell science 2021 Jul 1;134(13)
The Novel ALG-2 Target Protein CDIP1 Promotes Cell Death by Interacting with ESCRT-I and VAPA/B.
Inukai R, Mori K, Kuwata K, Suzuki C, Maki M, Takahara T, Shibata H
International journal of molecular sciences 2021 Jan 25;22(3)
International journal of molecular sciences 2021 Jan 25;22(3)
MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.
König T, Nolte H, Aaltonen MJ, Tatsuta T, Krols M, Stroh T, Langer T, McBride HM
Nature cell biology 2021 Dec;23(12):1271-1286
Nature cell biology 2021 Dec;23(12):1271-1286
VPS13D interacts with VCP/p97 and negatively regulates endoplasmic reticulum-mitochondria interactions.
Du Y, Wang J, Xiong J, Fang N, Ji WK
Molecular biology of the cell 2021 Aug 1;32(16):1474-1486
Molecular biology of the cell 2021 Aug 1;32(16):1474-1486
A High-Density Human Mitochondrial Proximity Interaction Network.
Antonicka H, Lin ZY, Janer A, Aaltonen MJ, Weraarpachai W, Gingras AC, Shoubridge EA
Cell metabolism 2020 Sep 1;32(3):479-497.e9
Cell metabolism 2020 Sep 1;32(3):479-497.e9
Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation.
Yamanaka T, Nishiyama R, Shimogori T, Nukina N
Scientific reports 2020 May 6;10(1):7610
Scientific reports 2020 May 6;10(1):7610
Nir2 Is an Effector of VAPs Necessary for Efficient Hepatitis C Virus Replication and Phosphatidylinositol 4-Phosphate Enrichment at the Viral Replication Organelle.
Wang H, Tai AW
Journal of virology 2019 Nov 15;93(22)
Journal of virology 2019 Nov 15;93(22)
A trap mutant reveals the physiological client spectrum of TRC40.
Coy-Vergara J, Rivera-Monroy J, Urlaub H, Lenz C, Schwappach B
Journal of cell science 2019 Jul 1;132(13)
Journal of cell science 2019 Jul 1;132(13)
MIGA2 Links Mitochondria, the ER, and Lipid Droplets and Promotes De Novo Lipogenesis in Adipocytes.
Freyre CAC, Rauher PC, Ejsing CS, Klemm RW
Molecular cell 2019 Dec 5;76(5):811-825.e14
Molecular cell 2019 Dec 5;76(5):811-825.e14
Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways.
Young PA, Senkal CE, Suchanek AL, Grevengoed TJ, Lin DD, Zhao L, Crunk AE, Klett EL, Füllekrug J, Obeid LM, Coleman RA
The Journal of biological chemistry 2018 Oct 26;293(43):16724-16740
The Journal of biological chemistry 2018 Oct 26;293(43):16724-16740
Loss of VAPB Regulates Autophagy in a Beclin 1-Dependent Manner.
Wu D, Hao Z, Ren H, Wang G
Neuroscience bulletin 2018 Dec;34(6):1037-1046
Neuroscience bulletin 2018 Dec;34(6):1037-1046
DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.
Zheng P, Chen Q, Tian X, Qian N, Chai P, Liu B, Hu J, Blackstone C, Zhu D, Teng J, Chen J
Cell research 2018 Aug;28(8):833-854
Cell research 2018 Aug;28(8):833-854
Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR.
Simonetti B, Danson CM, Heesom KJ, Cullen PJ
The Journal of cell biology 2017 Nov 6;216(11):3695-3712
The Journal of cell biology 2017 Nov 6;216(11):3695-3712
Adenovirus Modulates Toll-Like Receptor 4 Signaling by Reprogramming ORP1L-VAP Protein Contacts for Cholesterol Transport from Endosomes to the Endoplasmic Reticulum.
Cianciola NL, Chung S, Manor D, Carlin CR
Journal of virology 2017 Mar 15;91(6)
Journal of virology 2017 Mar 15;91(6)
Neuronal overexpression of human VAPB slows motor impairment and neuromuscular denervation in a mouse model of ALS.
Kim JY, Jang A, Reddy R, Yoon WH, Jankowsky JL
Human molecular genetics 2016 Nov 1;25(21):4661-4673
Human molecular genetics 2016 Nov 1;25(21):4661-4673
The ATF6 pathway of the ER stress response contributes to enhanced viability in glioblastoma.
Dadey DY, Kapoor V, Khudanyan A, Urano F, Kim AH, Thotala D, Hallahan DE
Oncotarget 2016 Jan 12;7(2):2080-92
Oncotarget 2016 Jan 12;7(2):2080-92
The role of endoplasmic reticulum-related BiP/GRP78 in interferon gamma-induced persistent Chlamydia pneumoniae infection.
Shima K, Klinger M, Schütze S, Kaufhold I, Solbach W, Reiling N, Rupp J
Cellular microbiology 2015 Jul;17(7):923-34
Cellular microbiology 2015 Jul;17(7):923-34
Expression of vesicle-associated membrane-protein-associated protein B cleavage products in peripheral blood leukocytes and cerebrospinal fluid of patients with sporadic amyotrophic lateral sclerosis.
Deidda I, Galizzi G, Passantino R, Cascio C, Russo D, Colletti T, La Bella V, Guarneri P
European journal of neurology 2014 Mar;21(3):478-85
European journal of neurology 2014 Mar;21(3):478-85
Widespread aggregation of mutant VAPB associated with ALS does not cause motor neuron degeneration or modulate mutant SOD1 aggregation and toxicity in mice.
Qiu L, Qiao T, Beers M, Tan W, Wang H, Yang B, Xu Z
Molecular neurodegeneration 2013 Jan 3;8:1
Molecular neurodegeneration 2013 Jan 3;8:1
Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation.
McCray BA, Skordalakes E, Taylor JP
Human molecular genetics 2010 Mar 15;19(6):1033-47
Human molecular genetics 2010 Mar 15;19(6):1033-47
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 14477-1-AP(VAPB antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using VAPB antibody 14477-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-VAPB (IP:14477-1-AP, 3ug; Detection:14477-1-AP 1:800) with HeLa cells lysate 1840ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human pancreas cancer using 14477-1-AP(VAPB antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human pancreas cancer using 14477-1-AP(VAPB antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The VAPB antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human VAPB. This antibody recognizes human,mouse,rat antigen. The VAPB antibody has been validated for the following applications: ELISA, WB, IHC, IF, IP, FC analysis.
