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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- MA5-24348 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VAPB Monoclonal Antibody (736904)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 736904
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of VAPB in HepG2 human hepatocellular carcinoma cell line, SH‚SY5Y human neuroblastoma cell line, and human heart tissue. Samples were incubated in VAPB monoclonal antibody (Product # MA5-24348) using a dilution of 0.1 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for VAP‚B at approximately 32-33 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of VAPB in immersion fixed paraffin-embedded sections of human brain (medulla). Samples were incubated in VAPB monoclonal antibody (Product # MA5-24348) using a dilution of 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic . Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of T98G human glioblastoma cell line was stained with mouse Anti-human VAP-B Monoclonal Antibody (Product # MA5-24348) or isotype control antibodyopen histogram), followed by Allophycocyanin-conjugated Anti-mouse IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehye and permeabilized with saponin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of VAPB in T98G human glioblastoma cell line. Samples were incubated in VAPB monoclonal antibody (Product # MA5-24348) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehye and permeabilized with saponin.
