MAB58551

antibody from R&D Systems

Targeting: VAPB ALS8, VAP-B, VAP-C

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB58551

Provider

R&D Systems

Product name

Human VAP-B Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human VAP-B in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human VAP-A is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

O95292


Isotype

IgG

Antibody clone number

736904

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human VAP-B by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, SH-SY5Y human neuroblastoma cell line, and human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human VAP-B Monoclonal Antibody (Catalog # MAB58551) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAP-B at approximately 32-33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

VAP-B in Human Brain. VAP-B was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human VAP-B Monoclonal Antibody (Catalog # MAB58551) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of VAPB in T98G Human Cell Line by Flow Cytometry. T98G human glioblastoma cell line was stained with Mouse Anti-Human VAP-B Monoclonal Antibody (Catalog # MAB58551, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehye and permeabilized with saponin.