Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-27582 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Alpha A Crystallin Monoclonal Antibody (1H3.B8)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- 0.5 µg/mL was sufficient for detection of 100 ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.|Detects approximately 20kDa. Does not cross-react with alpha B-crystallin, beta L-crystallin, beta H- crystallin, gamma -crystallin, HSP25, HSP27 or HSP47 proteins.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1H3.B8
- Vial size
- 200 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Alpha A Crystallin in bovine tissue lysate with 15 µg of sample. The sample was blocked with 1.5% BSA, incubated with Alpha A Crystallin monoclonal antibody (Product # MA5-27582) using a dilution of 1:1000 (2 hours at 4°C), followed by Left) Alpha A Crystallin and, Right) Alpha B Crystallin (1 hour at RT).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Alpha A Crystallin in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with Alpha A Crystallin monoclonal antibody (Product # MA5-27582) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) Alpha A Crystallin Antibody, D) Merged image. Magnification: 60x.
