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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-120942 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Alpha A Crystallin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive test controls include: Mouse kidney, Mouse liver, Mouse heart. The target is usually found in the following locations: Cytoplasm, Nucleus. Immunogen sequence: MDVTIQHPWF KRTLGPFYPS RLFDQFFGEG LFEYDLLPFL SSTISPYYRQ SLFRTVLDSG ISEVRSDRDK FVIFLDVKHF SPEDLTVKVQ DDFVEIHGKH NERQDDHGYI SREFHRRYRL PSNVDQSALS CSLSADGMLT FCGPKIQTGL DATHAERAIP VSREEKPTSA PSS
- Reactivity
- Human, Mouse, Rat, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.07 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Alpha A Crystallin in HepG2 cells. Samples were incubated with Alpha A Crystallin Polyclonal antibody (Product # PA5-120942) using a dilution of 1:5,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Alpha A Crystallin in HeLa cells using a Alpha A Crystallin Polyclonal antibody (Product # PA5-120942). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Immunofluorescence) analysis of Alpha A Crystallin in rat eye. Samples were incubated with Alpha A Crystallin Polyclonal antibody (Product # PA5-120942) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
