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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP11744PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP11744PU-N, RRID:AB_1747895
- Product name
- anti ACO2 (N-term)
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human Aconitase.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 1. Perfused isolated rat heart whole tissue lysate was lysed with either A) 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v% protease inhibitor cocktail or B) T-PER Tissue Protein Extraction Reagent, containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail; PVDF membrane was incubated in primary Ab [rabbit polyclonal antibody against ACO2 (N-term) (AP11744PU-N)]. Solution: 1:1000 diluted in 5% NFM TBS-T 0,05 for overnight (15 hrs) at 4ºC. Data courtesy of Boglarka Laczy M.D., Division of Cardiovascular Disease, Dept. of Medicine, University of Alabama at Birmingham.
