AP11745PU-N

antibody from Acris Antibodies GmbH

Targeting: ACO2 ACONM

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AP11745PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP11745PU-N, RRID:AB_1747892

Product name

anti ACO2

Antibody type

Polyclonal

Antigen

This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the central region of human Aconitase.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

0.4 ml

Concentration

lot specific

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis of anti-ACO2 (AP11745PU-N) in mouse heart (left) and 293 (right) tissue lysates (35 µg/lane). ACO2 (arrow) was detected using the purified Pab (1:60 dilution).

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Perfused isolated rat heart whole tissue lysate was lysed with either A) 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail or B) T-PER Tissue Protein Extraction Reagent, containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail; PVDF membrane was incubated in primary Ab [rabbit polyclonal antibody against ACO2 (Center) (AP11745PU-N). Solution: 1:1000 diluted in 5% NFM TBS-T 0,05 for overnight (15 hrs) at 4 ºC. Data courtesy of Boglarka Laczy M.D., Division of Cardiovascular Disease, Dept. of Medicine, University of Alabama at Birmingham.