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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- TA500841 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500841, RRID:AB_11124995
- Product name
- Anti-ACO2 (Aconitase 2) mouse monoclonal antibody, clone OTI7A11 (formerly 7A11)
- Antibody type
- Monoclonal
- Description
- Anti-ACO2 (Aconitase 2) mouse monoclonal antibody, clone OTI7A11 (formerly 7A11)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ACO2
- Isotype
- IgG
- Antibody clone number
- OTI7A11
- Vial size
- 100 µl
- Concentration
- 0.78 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACO2 (RC204307, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACO2.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-ACO2 mouse monoclonal antibody (TA500841) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACO2(RC204307).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either pCMV6-ENTRY ACO2(RC204307)(Red) or empty vector control plasmid(Blue) were immunostained with anti-ACO2 mouse monoclonal(TA500841), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-ACO2 antibody(TA500841),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
- Validation comment
- FC
