Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-19269 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Aconitase 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Posttranslational regulation of mitochondrial frataxin and identification of compounds that increase frataxin levels in Friedreich's ataxia.
Hackett PT, Jia X, Li L, Ward DM
The Journal of biological chemistry 2022 Jun;298(6):101982
The Journal of biological chemistry 2022 Jun;298(6):101982
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Aconitase 2 by a Aconitase 2 monoclonal antibody (Product # PA5-19269) at a concentration of 0.1 µg/mL. Human (A), Mouse (B) and Rat (C) Adipose lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human Heart lysate using Product # PA5-19269 at a concentration of 0.01 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Aconitase 2 by a Aconitase 2 monoclonal antibody (Product # PA5-19269) at a concentration of 0.01 µg/mL. Mouse (A), Rat (B) and Pig (C) Skeletal Muscle lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Aconitase 2 in paraffin embedded human breast using a Aconitase 2 polyclonal antibody (Product #PA5-19269) at a concentration of 3.8 µg/mL. Steamed antigen retrieval was performed with pH 6 buffered citrate. Samples were then stained with alkaline phosphatase.