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- References [1]
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- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- ABIN499115 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Aconitase 2, Mitochondrial (ACO2) (AA 541-555) antibody
- Antibody type
- Polyclonal
- Antigen
- Peptide with sequence from the internal region of the protein sequence according toNP_001089. 1. AA Sequence: C-QDTYQHPPKDSSGQH
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Porcine
- Host
- Goat
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Submitted references Lon protease preferentially degrades oxidized mitochondrial aconitase by an ATP-stimulated mechanism.
Bota DA, Davies KJ
Nature cell biology 2002 Sep;4(9):674-80
Nature cell biology 2002 Sep;4(9):674-80
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- AP21267PU-N ACO2 Antibody staining of Human (A), Mouse (B) and Rat (C) Adipose lysates at 0.1 μg/ml (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- AP21267PU-N ACO2 Antibody staining of Mouse (A), Rat (B) and Pig (C) Skeletal Muscle lysates at 0.1 μg/ml (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- AP21267PU-N ACO2 Antibody staining of Human Heart lysate at 0.01 μg/ml (35μg protein in RIPA buffer. Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- AP21267PU-N ACO2 Antibody staining of paraffin embedded Human Breast at 3.8 μg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
