15401-1-AP

antibody from Proteintech Group

Targeting: PPAT GPAT, PRAT

Provider product page for 15401-1-AP

Western blot

ELISA

Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [40]
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Validations
Western blot [1]

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Product number: 15401-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#15401-1-AP, RRID:AB_2166532
Product name: PPAT antibody
Antibody type: Polyclonal
Description: KD/KO validated PPAT antibody (Cat. #15401-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

PPAT protein structure - 15401-1-AP shown in red.

Submitted references Redox cycling nitroxide limits cellular iron availability and selectively inhibits iron-sulfur cluster metabolism.

Terzi EM, Fujihara KM, Molenaars M, Biancur DE, Possemato R
Cell death discovery 2026 Mar 24;12(1)

Investigation of the effects and underlying mechanisms of adenylate kinase 1 on inosine monophosphate deposition in Beijing-You chickens.

Hou J, Zhang Y, Zhu L, Qi X, Wang X, Lin Z, Xiao L, Long C, Dou J, Xu Y, Sheng X
Poultry science 2026 Mar 23;105(6):106828

De novo purine synthesis reprograms the macrophage inflammatory response and the immune response in sepsis.

Haskó G, Liu L, Németh ZH, Wagener G, Akcan U, Arif M, Pacher P, Kelestemur T
Research square 2026 Feb 19;

LEDGF Binds H3R17me2a Promoting De Novo Nucleotide Biosynthesis in SETD2 Mutant Clear Cell Renal Cell Carcinoma.

Zhang Y, Zhou Y, Zhang Y, Lv J, Shen Y, Zhang D, Liu B, Zhao W, Ju J, Zhu Q, Wang K, Feng N
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Sep;12(35):e16809

Oxyberberine alleviates fructose-induced hyperuricemia by modulating purine metabolism and gut microbiota.

Tan W, Xie X, Yu Q, Yan G, Chen H, Xie Q, Liu Y, Huang X, Chen J, Xie J, Su Z, Li Y
European journal of pharmacology 2025 Oct 15;1005:178061

Uridine-sensitized screening identifies demethoxy-coenzyme Q and NUDT5 as regulators of nucleotide synthesis.

Strefeler A, Baker ZN, Chollet S, Foged MM, Guerra RM, Ivanisevic J, Gallart-Ayala H, Pagliarini DJ, Jourdain AA
Nature metabolism 2025 Nov;7(11):2221-2235

Adaptive nuclear GTP synthesis promotes glioblastoma treatment resistance and is a targetable vulnerability in patients.

Scott AJ, Liang N, Al-Holou WN, Xu J, O'Brien A, Lin A, Pareek V, Sha Z, Zhao Z, Yang JZ, Yang A, Kothari AU, Wilder-Romans K, Wu Z, Feng J, Korimerla N, Elaimy A, Palavalasa S, Chen J, Wang L, Zhang L, Andren AC, Sajjakulnukit P, Marini B, Heth JA, Morgan MA, Lawrence TS, Benkovic SJ, Nagrath D, Chandrasekaran S, Qi NR, Sun D, Wen B, Pai MP, Clarke N, Suresh K, Umemura Y, Lyssiotis CA, Zhou W, Wahl DR
medRxiv : the preprint server for health sciences 2025 Nov 13;

Mechanistic insights into metformin's anti-hyperuricemic effect: Targeting PPP/DNPB/XOD-mediated purine pathway, purinosome assembly, and gut microbiota homostasis in rats.

Yu Q, Liao H, Tan W, Xu L, Lin G, Ai Z, Xie Q, Liu Y, Li Y, Yang X, Su Z, Ma G, Xie J, Lai Z
Chemico-biological interactions 2025 Nov 1;421:111783

Patient-derived organotypic tissue cultures as a platform to evaluate metabolic reprogramming in breast cancer patients.

Fan TW, Yan J, Goncalves CFL, Islam JMM, Lin P, Kaddah MMY, Higashi RM, Lane AN, Wang X, Zhu C
The Journal of biological chemistry 2025 May;301(5):108495

Uridine-sensitized screening identifies genes and metabolic regulators of nucleotide synthesis.

Strefeler A, Baker ZN, Chollet S, Guerra RM, Ivanisevic J, Gallart-Ayala H, Pagliarini DJ, Jourdain AA
bioRxiv : the preprint server for biology 2025 Mar 13;

A non-enzymatic role of Nudix hydrolase 5 in repressing purine de novo synthesis.

Nguyen TA, Lin JG, Marques AMC, Fottner M, Bauer LG, Reicher A, Daum D, Scrofani L, Liu Y, Cheng C, D'Angelo L D D L, Sanchez J, Bueschl C, Marella N, Buphamalai P, Traversi F, Bereš M, Moll HP, Siklos M, Genger JW, Hofstaetter G, Villanti L, Malik M, Klimek C, Runggatscher K, Guertl B, Hansen JS, Dobner S, Babosova O, Becirovic T, de Rooij LPMH, Casanova E, Koren A, Froese DS, Rosenblatt DS, Klavins K, Bergthaler A, Menche J, Hannich JT, Abele M, Sdelci S, Lang K, Huber KVM, Kubicek S
Science (New York, N.Y.) 2025 Dec 11;390(6778):1143-1150

NUDT5 regulates purine metabolism and thiopurine sensitivity by interacting with PPAT.

Wu Z, Nguyen PT, Sondhi V, Yao RW, Lu Z, Dai T, Chiang JC, Cai F, Williams IM, Blatt EB, Shang Z, Cai L, Zhang J, Moore MD, Alshamleh I, Li X, Ogu T, Zacharias LG, Winston R, Patricio JS, Johnson X, Chen WM, Cong Q, Mathews TP, Zhang Y, Zhang L, DeBerardinis RJ
Science (New York, N.Y.) 2025 Dec 11;390(6778):1134-1142

Prolonged Fructose Overconsumption Activates Original Biosynthetic and Metabolic Pathways of Endogenous Purine in Rats.

Yu Q, Tan W, Ma X, Xu L, Lin G, Cheng J, Xie Q, Liu Y, Li Y, Xian Y, Lin Z, Chen J, Su Z, Yang X, Xie J
Molecular nutrition & food research 2025 Aug;69(16):e70122

Phosphoribosyl pyrophosphate amidotransferase: Novel biomarker and therapeutic target for nasopharyngeal carcinoma.

Kitagawa Y, Kondo S, Fukuyo M, Wakae K, Dochi H, Mizokami H, Komura S, Kobayashi E, Hirai N, Ueno T, Nakanishi Y, Endo K, Sugimoto H, Wakisaka N, Kaneda A, Yoshizaki T
Cancer science 2024 Nov;115(11):3587-3595

Mitochondrial respiratory function is preserved under cysteine starvation via glutathione catabolism in NSCLC.

Ward NP, Yoon SJ, Flynn T, Sherwood AM, Olley MA, Madej J, DeNicola GM
Nature communications 2024 May 18;15(1):4244

Lactobacillus rhamnosus GG ameliorates hyperuricemia in a novel model.

Fu Y, Chen YS, Xia DY, Luo XD, Luo HT, Pan J, Ma WQ, Li JZ, Mo QY, Tu Q, Li MM, Zhao Y, Li Y, Huang YT, Chen ZX, Li ZJ, Bernard L, Dione M, Zhang YM, Miao K, Chen JY, Zhu SS, Ren J, Zhou LJ, Jiang XZ, Chen J, Lin ZP, Chen JP, Ye H, Cao QY, Zhu YW, Yang L, Wang X, Wang WC
NPJ biofilms and microbiomes 2024 Mar 20;10(1):25

CIAO1 loss of function causes a neuromuscular disorder with compromise of nucleocytoplasmic Fe-S enzymes.

Maio N, Orbach R, Zaharieva IT, Töpf A, Donkervoort S, Munot P, Mueller J, Willis T, Verma S, Peric S, Krishnakumar D, Sudhakar S, Foley AR, Silverstein S, Douglas G, Pais L, DiTroia S, Grunseich C, Hu Y, Sewry C, Sarkozy A, Straub V, Muntoni F, Rouault TA, Bönnemann CG
The Journal of clinical investigation 2024 Jun 17;134(12)

De novo and salvage purine synthesis pathways across tissues and tumors.

Tran DH, Kim D, Kesavan R, Brown H, Dey T, Soflaee MH, Vu HS, Tasdogan A, Guo J, Bezwada D, Al Saad H, Cai F, Solmonson A, Rion H, Chabatya R, Merchant S, Manales NJ, Tcheuyap VT, Mulkey M, Mathews TP, Brugarolas J, Morrison SJ, Zhu H, DeBerardinis RJ, Hoxhaj G
Cell 2024 Jul 11;187(14):3602-3618.e20

The Role of Purine Metabolism-Related Genes PPAT and IMPDH1 in the Carcinogenesis of Intrahepatic Cholangiocarcinoma Based on Metabonomic and Bioinformatic Analyses.

Liu CJ, Ma ZZ, Gong WZ, Mao XH, Wen HQ, Wang XH
Journal of oncology 2023;2023:5141836

Bi-steric mTORC1 inhibitors induce apoptotic cell death in tumor models with hyperactivated mTORC1.

Du H, Yang YC, Liu HJ, Yuan M, Asara JM, Wong KK, Henske EP, Singh M, Kwiatkowski DJ
The Journal of clinical investigation 2023 Nov 1;133(21)

A mitochondrial iron-responsive pathway regulated by DELE1.

Sekine Y, Houston R, Eckl EM, Fessler E, Narendra DP, Jae LT, Sekine S
Molecular cell 2023 Jun 15;83(12):2059-2076.e6

Cyclin D1 extensively reprograms metabolism to support biosynthetic pathways in hepatocytes.

Wu H, Kren BT, Lane AN, Cassel TA, Higashi RM, Fan TWM, Scaria GS, Shekels LL, Klein MA, Albrecht JH
The Journal of biological chemistry 2023 Dec;299(12):105407

Therapeutic targeting of glutamate dehydrogenase 1 that links metabolic reprogramming and Snail-mediated epithelial-mesenchymal transition in drug-resistant lung cancer.

Wang Q, Wu M, Li H, Rao X, Ao L, Wang H, Yao L, Wang X, Hong X, Wang J, Aa J, Sun M, Wang G, Liu J, Zhou F
Pharmacological research 2022 Nov;185:106490

SHMT2 promotes the tumorigenesis of renal cell carcinoma by regulating the m6A modification of PPAT.

Huo FC, Xie M, Zhu ZM, Zheng JN, Pei DS
Genomics 2022 Jul;114(4):110424

Species-Specific Deamidation of RIG-I Reveals Collaborative Action between Viral and Cellular Deamidases in HSV-1 Lytic Replication.

Huang H, Zhao J, Wang TY, Zhang S, Zhou Y, Rao Y, Qin C, Liu Y, Chen Y, Xia Z, Feng P
mBio 2021 Mar 30;12(2)

Targeting Subtype-Specific Metabolic Preferences in Nucleotide Biosynthesis Inhibits Tumor Growth in a Breast Cancer Model.

Ogrodzinski MP, Teoh ST, Lunt SY
Cancer research 2021 Jan 15;81(2):303-314

Mitochondrial NADP(+) is essential for proline biosynthesis during cell growth.

Tran DH, Kesavan R, Rion H, Soflaee MH, Solmonson A, Bezwada D, Vu HS, Cai F, Phillips JA 3rd, DeBerardinis RJ, Hoxhaj G
Nature metabolism 2021 Apr;3(4):571-585

UHMK1 promotes gastric cancer progression through reprogramming nucleotide metabolism.

Feng X, Ma D, Zhao J, Song Y, Zhu Y, Zhou Q, Ma F, Liu X, Zhong M, Liu Y, Xiong Y, Qiu X, Zhang Z, Zhang H, Zhao Y, Zhang K, Hong X, Zhang Z
The EMBO journal 2020 Mar 2;39(5):e102541

A shift in glutamine nitrogen metabolism contributes to the malignant progression of cancer.

Kodama M, Oshikawa K, Shimizu H, Yoshioka S, Takahashi M, Izumi Y, Bamba T, Tateishi C, Tomonaga T, Matsumoto M, Nakayama KI
Nature communications 2020 Mar 17;11(1):1320

ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.

Ali ES, Sahu U, Villa E, O'Hara BP, Gao P, Beaudet C, Wood AW, Asara JM, Ben-Sahra I
Molecular cell 2020 Jun 18;78(6):1178-1191.e6

Sideroflexin 4 affects Fe-S cluster biogenesis, iron metabolism, mitochondrial respiration and heme biosynthetic enzymes.

Paul BT, Tesfay L, Winkler CR, Torti FM, Torti SV
Scientific reports 2019 Dec 23;9(1):19634

Effect and Mechanism of Tanshinone I on the Radiosensitivity of Lung Cancer Cells.

Yan Y, Su W, Zeng S, Qian L, Chen X, Wei J, Chen N, Gong Z, Xu Z
Molecular pharmaceutics 2018 Nov 5;15(11):4843-4853

The mTORC1 Signaling Network Senses Changes in Cellular Purine Nucleotide Levels.

Hoxhaj G, Hughes-Hallett J, Timson RC, Ilagan E, Yuan M, Asara JM, Ben-Sahra I, Manning BD
Cell reports 2017 Oct 31;21(5):1331-1346

Menin enhances c-Myc-mediated transcription to promote cancer progression.

Wu G, Yuan M, Shen S, Ma X, Fang J, Zhu L, Sun L, Liu Z, He X, Huang D, Li T, Li C, Wu J, Hu X, Li Z, Song L, Qu K, Zhang H, Gao P
Nature communications 2017 May 5;8:15278

mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.

Ben-Sahra I, Hoxhaj G, Ricoult SJH, Asara JM, Manning BD
Science (New York, N.Y.) 2016 Feb 12;351(6274):728-733

Aripiprazole increases NAD(P)H-quinone oxidoreductase-1 and heme oxygenase-1 in PC12 cells.

Kaneko YS, Takayanagi T, Nagasaki H, Kodani Y, Nakashima A, Mori K, Suzuki A, Itoh M, Kondo K, Nagatsu T, Ota M, Ota A
Journal of neural transmission (Vienna, Austria : 1996) 2015 Jun;122(6):757-72

Potent effects of dioscin against obesity in mice.

Liu M, Xu L, Yin L, Qi Y, Xu Y, Han X, Zhao Y, Sun H, Yao J, Lin Y, Liu K, Peng J
Scientific reports 2015 Jan 22;5:7973

Prominent steatosis with hypermetabolism of the cell line permissive for years of infection with hepatitis C virus.

Sugiyama K, Ebinuma H, Nakamoto N, Sakasegawa N, Murakami Y, Chu PS, Usui S, Ishibashi Y, Wakayama Y, Taniki N, Murata H, Saito Y, Fukasawa M, Saito K, Yamagishi Y, Wakita T, Takaku H, Hibi T, Saito H, Kanai T
PloS one 2014;9(4):e94460

Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.

Dong D, Qi Y, Xu L, Yin L, Xu Y, Han X, Zhao Y, Peng J
Food & function 2014 Dec;5(12):3065-3075

Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming.

Mitsuishi Y, Taguchi K, Kawatani Y, Shibata T, Nukiwa T, Aburatani H, Yamamoto M, Motohashi H
Cancer cell 2012 Jul 10;22(1):66-79

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The PPAT antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human PPAT. This antibody recognizes human, mouse, rat antigen. The PPAT antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 15401-1-AP.pdf

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