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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00005471-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005471-D01, RRID:AB_10720717
- Product name
- PPAT MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human PPAT protein.
- Antigen sequence
MELEELGIREECGVFGCIASGEWPTQLDVPHVITL
GLVGLQHRGQESAGIVTSDGSSVPTFKSHKGMGLV
NHVFTEDNLKKLYVSNLGIGHTRYATTGKCELENC
QPFVVETLHGKIAVAHNGELVNAARLRKKLLRHGI
GLSTSSDSEMITQLLAYTPPQEQDDTPDWVARIKN
LMKEAPTAYSLLIMHRDVIYAVRDPYGNRPLCIGR
LIPVSDINDKEKKTSETEGWVVSSESCSFLSIGAR
YYREVLPGEIVEISRHNVQTLDIISRSEGNPVAFC
IFEYVYFARPDSMFEDQMVYTVRYRCGQQLAIEAP
VDADLVSTVPESATPAALAYAGKCGLPYVEVLCKN
RYVGRTFIQPNMRLRQLGVAKKFGVLSDNFKGKRI
VLVDDSIVRGNTISPIIKLLKESGAKEVHIRVASP
PIKYPCFMGINIPTKEELIANKPEFDHLAEYLGAN
SVVYLSVEGLVSSVQEGIKFKKQKEKKHDIMIQEN
GNGLECFEKSGHCTACLTGKYPVELEW- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PPAT expression in transfected 293T cell line () by PPAT MaxPab polyclonal antibody.Lane 1: PPAT transfected lysate(57.40 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of PPAT transfected lysate using anti-PPAT MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PPAT purified MaxPab mouse polyclonal antibody (B01P) (H00005471-B01P).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
