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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00003108-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003108-M01, RRID:AB_425480
- Product name
- HLA-DMA monoclonal antibody (M01), clone 3F12-F11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant HLA-DMA.
- Antigen sequence
MGHEQNQGAALLQMLPLLWLLPHSWAVPEAPTPMW
PDDLQNHTFLHTVYCQDGSPSVGLSEAYDEDQLFF
FDFSQNTRVPRLPEFADWAQEQGDAPAILFDKEFC
EWMIQQIGPKLDGKIPVSRGFPIAEVFTLKPLEFG
KPNTLVCFVSNLFPPMLTVNWQHHSVPVEGFGPTF
VSAVDGLSFQAFSYLNFTPEPSDIFSCIVTHEIDR
YTAIAYWVPRNALPSDLLENVLCGVAFGLGVLGII
VGIVLIIYFRKPCSGD- Isotype
- IgG
- Antibody clone number
- 3F12-F11
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HLA-DMA monoclonal antibody (M01), clone 3F12-F11 Western Blot analysis of HLA-DMA expression in U-2 OS ( Cat # L022V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged HLA-DMA is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to HLA-DMA on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
