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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-22365 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HLA-DMA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HepG2, Jurkat, Raji. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HLA-DMA Polyclonal Antibody (Product # PA5-22365). Sample (30 µg of whole cell lysate). Lane A: Hela. 12% SDS PAGE. HLA-DMA Polyclonal Antibody (Product # PA5-22365) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HLA-DMA Polyclonal Antibody detects HLA-DMA protein by Western blot analysis. A. 30 µg Jurkat whole cell lysate/extract. B. 30 µg Raji whole cell lysate/extract.12 % SDS-PAGE. HLA-DMA Polyclonal Antibody (Product # PA5-22365) dilution: 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using HLA-DMA (Product # PA5-22365) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
