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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
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- Product number
- AF7558 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat eIF5A Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse and rat eIF5A in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P63241- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat eIF5A by Western Blot. Western blot shows lysates of 786-O human renal cell adenocarcinoma cell line, C2C12 mouse myoblast cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat eIF5A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7558) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for eIF5A at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- eIF5A in Rat Brain. eIF5A was detected in perfusion fixed frozen sections of rat brain (brainstem medulla) using Sheep Anti-Human/Mouse/Rat eIF5A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7558) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- eIF5A in Human Liver. eIF5A was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human/Mouse/Rat eIF5A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7558) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- eIF5A in Mouse Embryo. eIF5A was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Human/Mouse/Rat eIF5A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7558) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing central nervous system. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
