Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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Validation data
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- Product number
- H00000828-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000828-M01, RRID:AB_537394
- Product name
- Mouse Monoclonal Calcyphosine Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CAPS - calcyphosine
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Identification of novel biomarkers in pediatric primitive neuroectodermal tumors and ependymomas by proteome-wide analysis.
de Bont JM, den Boer ML, Kros JM, Passier MM, Reddingius RE, Smitt PA, Luider TM, Pieters R
Journal of neuropathology and experimental neurology 2007 Jun;66(6):505-16
Journal of neuropathology and experimental neurology 2007 Jun;66(6):505-16
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Calcyphosine Antibody (4C6) [H00000828-M01] - Analysis of CAPS over-expressed 293 cell line, cotransfected with CAPS Validated Chimera RNAi ( Cat # H00000828-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CAPS monoclonal antibody (M01), clone 4C6 (Cat # H00000828-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Calcyphosine Antibody (4C6) [H00000828-M01] - Analysis of CAPS expression in transfected 293T cell line by CAPS monoclonal antibody (M01), clone 4C6.Lane 1: CAPS transfected lysate(21 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Calcyphosine Antibody (4C6) [H00000828-M01] - Detection limit for recombinant GST tagged CAPS is approximately 0.03ng/ml as a capture antibody.