TA501469

antibody from OriGene

Targeting: PAICS ADE2H1, AIRC, PAIS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501469

Provider

OriGene

Proper citation

OriGene Cat#TA501469, RRID:AB_11126015

Product name

Anti-PAICS mouse monoclonal antibody, clone OTI5B6 (formerly 5B6)

Antibody type

Monoclonal

Description

Anti-PAICS mouse monoclonal antibody, clone OTI5B6 (formerly 5B6)

Host

Mouse

Conjugate

Unconjugated

Epitope

PAICS

Isotype

IgG

Antibody clone number

OTI5B6

Vial size

100 µl

Concentration

NULL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAICS (RC215225, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAICS.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-PAICS monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-PAICS mouse monoclonal antibody (TA501469) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PAICS(RC215225).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC215225 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PAICS antibody(TA501469), and then analyzed by flow cytometry.

Validation comment

FC