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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-92985 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PAICS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LEKSWLPQNC TLVDMKIEFG VDVTTKEIVL ADVIDNDSWR LWPSGDRSQQ KDKQSYRDLK EVTPEGLQMV KKNFEWVAER VELLLKSESQ CRVVVLMGST SDLGHCEKIK KACGNFGIPC ELRVTSAHKG PDETLRIKAE YEGDGIPTVF VAVAGRSNGL GPVMSGNTAY PVISCPPLTP DWGVQDVWSS LRLPSGLGCS TVLSPEGSAQ FAAQIFGLSN HLVWSKLRAS ILNTWISLKQ ADKKIRECNL; Positive Samples: Jurkat, 293T, SW620, Raji, HeLa
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.64 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using PAICS Polyclonal antibody (Product # PA5-92985) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PAICS in extracts of various cell lines using PAICS Polyclonal Antibody (Product # PA5-92985) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PAICS was performed in U2OS cells using PAICS Polyclonal Antibody (Product # PA5-92985).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PAICS was performed in U2OS cells using PAICS Polyclonal Antibody (Product # PA5-92985).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PAICS in paraffin-embedded rat spleen using PAICS Polyclonal Antibody (Product # PA5-92985) at a dilution of 1:200.
