12967-1-AP

antibody from Invitrogen Antibodies

Targeting: PAICS ADE2H1, AIRC, PAIS

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

12967-1-AP

Provider

Invitrogen Antibodies

Product name

PAICS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: AFTRKCGET AFIAPQCEMI PIEWVCRRIA TGSFLKRNPG VKEGYKFYPP KVELFFKDDA NNDPQWSEEQ LIAAKFCFAG LLIGQTEVDI MSHATQAIFE ILEKSWLPQN CTLVDMKIEF GVDVTTKEIV LADVIDNDSW RLWPSGDRSQ QKDKQSYRDL KEVTPEGLQM VKKNFEWVAE RVELLLKSES QCRVVVLMGS TSDLGHCEKI KKACGNFGIP CELRVTSAHK GPDETLRIKA EYEGDGIPTV FVAVAGRSNG LGPVMSGNTA YPVISCPPLT PDWGVQDVWS SLRLPSGLGC STVLSPEGSA QFAAQIFGLS NHLVWSKLRA SILNTWISLK QADKKIRECN L (76-425 aa encoded by BC010273)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.17 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 12967-1-AP (PAICS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 12967-1-AP (PAICS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Raji cells were subjected to SDS PAGE followed by western blot with 12967-1-AP (PAICS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis using 12967-1-AP (PAICS antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis using 12967-1-AP (PAICS antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver using 12967-1-AP (PAICS antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver using 12967-1-AP (PAICS antibody) at dilution of 1:50 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HeLa cells were stained with 0.2ug PAICS antibody (12967-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.