Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB84671-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human BTN1A1/Butyrophilin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human BTN1A1/Butyrophilin in direct ELISAs.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
Q13410
- Isotype
- IgG
- Antibody clone number
- 2151B
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- BTN1A1/Butyrophilin in HEK293 Human Cell Line. BTN1A1/Butyrophilin was detected in immersion fixed HEK293 human embryonic kidney cell line using Rabbit Anti-Human BTN1A1/Butyrophilin Polyclonal Antibody (Catalog # MAB84671) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of BTN1A1 in HEK293 Human Cell Line Transfected with Human BTN1A1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human BTN1A1 or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human BTN1A1 Monoclonal Antibody (Catalog # MAB84671) followed by APC-conjugated Goat-anti Rabbit IgG secondary antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.