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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-34972 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATG14 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HeLa, HepG2, 293T, RD, mouse brain, rat brain.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ATG14 was performed by separating 60 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ATG14 Polyclonal Antibody (Product # PA5-34972) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATG14 Polyclonal Antibody detects ATG14 protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract. B. 30 µg HepG2 whole cell lysate/extract.10 % SDS-PAGE. ATG14 Polyclonal Antibody (Product # PA5-34972) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATG14 Polyclonal Antibody detects ATG14 protein at cytosol on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. ATG14 Polyclonal Antibody (Product # PA5-34972) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
