710702

antibody from Invitrogen Antibodies

Targeting: ATG14 ATG14L, KIAA0831

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

710702

Provider

Invitrogen Antibodies

Product name

ATG14 Recombinant Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (50 µg lysate) of HeLa serum starved and treated with Rapamycin (100nM/4 hours) (Lane1), HeLa Serum starved (Lane2), HeLa (Lane 3), A549 serum starved treated with Rapamycin (100nM/4 hours) (Lane 4), A549 serum starved (Lane5), A549 (Lane6), U-87-MG serum starved and treated with Rapamycin (100nM/4 hours) (Lane7) and U-87-MG (Lane8). The blots were probed with Anti- ATG 14 Recombinant Rabbit Polyclonal Antibody (Product # 710702, 1-3 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A clear 65kDa band corresponding to ATG14 was observed across cell lines according to the given treatment. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).