TA503265

antibody from OriGene

Targeting: SSR1 TRAPA

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA503265

Provider

OriGene

Proper citation

OriGene Cat#TA503265, RRID:AB_11123967

Product name

SSR1 (TRAP alpha) mouse monoclonal antibody, clone OTI 5D5 (formerly 5D5)

Antibody type

Monoclonal

Description

SSR1 (TRAP alpha) mouse monoclonal antibody, clone OTI 5D5 (formerly 5D5)

Host

Mouse

Conjugate

Unconjugated

Epitope

SSR1

Isotype

IgG

Antibody clone number

OTI 5D5

Vial size

100 µl

Concentration

0.29 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSR1 (RC202408, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSR1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SSR1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC202408 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SSR1 antibody(TA503265), and then analyzed by flow cytometry.

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Hela cells, using anti-SSR1 antibody(TA503265),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC