MA1-23277

antibody from Invitrogen Antibodies

Targeting: RBBP7 RbAp46

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-23277

Provider

Invitrogen Antibodies

Product name

RbAp46/RbAp48 Monoclonal Antibody (15G12)

Antibody type

Monoclonal

Antigen

Other

Description

Recommended positive controls: K562, THP-1, HL-60. Predicted molecular weight is 47.6kDa. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

15G12

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RbAp46, 48 using 30 µg of A) Raji (B) K562 (C) THP-1 and D) HL-60 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a RbAp46, 48 monoclonal antibody (Product # MA1-23277) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RbAp46/RbAp48 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RbAp46/RbAp48 Monoclonal Antibody (15G12) (Product # MA1-23277) at a dilution of 1:500 and a HRP-conjugated anti-mouse IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using RbAp46/RbAp48 Monoclonal Antibody (15G12) (Product # MA1-23277). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp46/RbAp48 Monoclonal Antibody (15G12) (Product # MA1-23277) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of RbAp46/RbAp48 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RbAp46/RbAp48 Monoclonal Antibody (15G12) (Product # MA1-23277) diluted at 1:500. Red: phalloidin, a cytoskeleton marker.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of RbAp46/RbAp48 was performed in paraffin-embedded human cervical carcinoma tissue using RbAp46/RbAp48 Monoclonal Antibody (15G12) (Product # MA1-23277) at a dilution of 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using RbAp48 + RbAp46 (Product # MA1-23277) antibody at 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using RbAp48 + RbAp46 (Product # MA1-23277) antibody at 1:200 dilution.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 ANRIL binds to PRC1 and 2 proteins and recruits CBX7 and SUZ12 to promoters of target genes. (A, B) RNA immunoprecipitation (RIP) followed by qRT-PCR demonstrating ANRIL binding to PRC but not to CoREST/REST proteins in (A) ANRIL2 and (B) ANRIL4 cells. Copies of ANRIL relative to input control are given in (A) blue and (B) red, nuclear ncRNA U1 (white) was used as negative control. rIgG/mIgG/gIgG- rabbit/mouse/goat IgG controls. Error bars indicate s.e.m. (C,D) SUZ12 binding in promoters of ANRIL up-(green), down-(red), and not (black) regulated genes in (C) vector control cell line and (D) in BGO3 cells (GSM602674). TSS- transcription start site. (E, F) Effect of ANRIL over-expression on (E) SUZ12 and (F) CBX7 binding in promoters of up-regulated genes (vector control- dotted line vs. ANRIL2- straight line). (G) Reversal of ANRIL trans -regulation by RNAi against SUZ12 and CBX7 in ANRIL2 cells. SCR- scrambled siRNA control.