Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A300-959A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RbBP7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human RbBP7 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RbBP7 antibody (Product # A300-959A; lot A300-959A-1) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human RbBP7 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RbBP7 antibody A300-959A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). RbBP7 was also immunoprecipitated by rabbit anti-RbBP7 antibody A300-958A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse RbBP7 by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- RbBP7 (Cat. No. A300-959A Lot1) used at a dilution of 1:1,000 (0.2µg/ml) and 1:200 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human RbBP7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RbBP7 antibody (Product # A300-959A; lot A300-959A-1) used for IP at 3 µg/mg lysate. RbBP7 was also immunoprecipitated by rabbit anti-RbBP7 antibody (Product # A300-958A), which recognizes an upstream epitope. For blotting immunoprecipitated RbBP7 (Product # A300-959A) was used at 1.0 µg/mL. Detection: Chemiluminescence with exposure time of 30 seconds.
