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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA1-868 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RbAp46 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-868 detects retinoblastoma protein (Rb) associated protein 46 (RbAp46) from human cells. PA1-868 has been successfully used in IF, Western blot and immunocytochemical procedures. By Western blot, this antibody detects an ~46 kDa protein representing RbAp46 from HeLa cell lysate. Immunocytochemical staining of RbAp46 in HeLa cells with PA1-868 results in nuclear staining. The PA1-868 immunogen is a synthetic peptide corresponding to residues M(1) A S K E M F E D T V E E(13) C of human RbAp46. This sequence is completely conserved between human and mouse. This immunizing peptide (Cat. # PEP-083) is available for use in neutralization and control experiments.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references A polycomb group protein, PHF1, is involved in the response to DNA double-strand breaks in human cell.
Atrophin 2 recruits histone deacetylase and is required for the function of multiple signaling centers during mouse embryogenesis.
Induction of Rb-associated protein (RbAp46) by Wilms' tumor suppressor WT1 mediates growth inhibition.
Induction of Rb-associated protein (RbAp46) by Wilms' tumor suppressor WT1 mediates growth inhibition.
Hong Z, Jiang J, Lan L, Nakajima S, Kanno S, Koseki H, Yasui A
Nucleic acids research 2008 May;36(9):2939-47
Nucleic acids research 2008 May;36(9):2939-47
Atrophin 2 recruits histone deacetylase and is required for the function of multiple signaling centers during mouse embryogenesis.
Zoltewicz JS, Stewart NJ, Leung R, Peterson AS
Development (Cambridge, England) 2004 Jan;131(1):3-14
Development (Cambridge, England) 2004 Jan;131(1):3-14
Induction of Rb-associated protein (RbAp46) by Wilms' tumor suppressor WT1 mediates growth inhibition.
Guan LS, Rauchman M, Wang ZY
The Journal of biological chemistry 1998 Oct 16;273(42):27047-50
The Journal of biological chemistry 1998 Oct 16;273(42):27047-50
Induction of Rb-associated protein (RbAp46) by Wilms' tumor suppressor WT1 mediates growth inhibition.
Guan LS, Rauchman M, Wang ZY
The Journal of biological chemistry 1998 Oct 16;273(42):27047-50
The Journal of biological chemistry 1998 Oct 16;273(42):27047-50
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of RbAp46 on 1) Du-145 2) LNCaP and 3) Hep-G2 cell extracts using Product # PA1-868.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunolocalization of RbAp46 in HeLa cells using Product # PA1-868.
