BAF496
antibody from Novus Biologicals
Targeting: EFNB2
EPLG5, Htk-L, HTKL, LERK5, MGC126226, MGC126227, MGC126228
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- BAF496 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Ephrin-B2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Ephrin-B2 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Ephrin-A5 is observed and less than 1% cross-reactivity with recombinant mouse Ephrin-B1 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references EphB4 forward signalling regulates lymphatic valve development.
Emerging pulmonary vasculature lacks fate specification.
Zhang G, Brady J, Liang WC, Wu Y, Henkemeyer M, Yan M
Nature communications 2015 Apr 13;6:6625
Nature communications 2015 Apr 13;6:6625
Emerging pulmonary vasculature lacks fate specification.
Schwarz MA, Caldwell L, Cafasso D, Zheng H
American journal of physiology. Lung cellular and molecular physiology 2009 Jan;296(1):L71-81
American journal of physiology. Lung cellular and molecular physiology 2009 Jan;296(1):L71-81
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Ephrin-B2 in Rat Brain. Ephrin-B2 was detected in perfusion fixed frozen sections of rat brain (trigeminal ganglia) using Goat Anti-Mouse Ephrin-B2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF496) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.