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antibody from R&D Systems
Targeting: EFNB2
EPLG5, Htk-L, HTKL, LERK5, MGC126226, MGC126227, MGC126228
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- BAF496 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Ephrin-B2 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Ephrin-B2 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Ephrin-A5 is observed and less than 1% cross-reactivity with recombinant mouse Ephrin-B1 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
AAA82934- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references EphB4 forward signalling regulates lymphatic valve development.
Emerging pulmonary vasculature lacks fate specification.
Zhang G, Brady J, Liang WC, Wu Y, Henkemeyer M, Yan M
Nature communications 2015 Apr 13;6:6625
Nature communications 2015 Apr 13;6:6625
Emerging pulmonary vasculature lacks fate specification.
Schwarz MA, Caldwell L, Cafasso D, Zheng H
American journal of physiology. Lung cellular and molecular physiology 2009 Jan;296(1):L71-81
American journal of physiology. Lung cellular and molecular physiology 2009 Jan;296(1):L71-81
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Ephrin-B2 in Rat Hippocampal Neurons. Ephrin-B2 was detected in immersion fixed rat hippocampal neurons using Goat Anti-Mouse Ephrin-B2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF496) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Ephrin-B2 in Rat Brain. Ephrin-B2 was detected in perfusion fixed frozen sections of rat brain (trigeminal ganglia) using Goat Anti-Mouse Ephrin-B2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF496) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
