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LearnAF496
antibody from R&D Systems
Targeting: EFNB2
EPLG5, Htk-L, HTKL, LERK5, MGC126226, MGC126227, MGC126228
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- AF496 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Ephrin-B2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Ephrin-B2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse Ephrin-B1 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
AAA82934- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Inhibition of Ephrin-B2 in brain pericytes decreases cerebral pathological neovascularization in diabetic rats.
EPHB6 controls catecholamine biosynthesis by up-regulating tyrosine hydroxylase transcription in adrenal gland chromaffin cells.
Histochemical assessment for osteoblastic activity coupled with dysfunctional osteoclasts in c-src deficient mice.
Embryonic Stem Cell Differentiation to Functional Arterial Endothelial Cells through Sequential Activation of ETV2 and NOTCH1 Signaling by HIF1α.
EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance.
Tissue-Specific Effects of Reduced β-catenin Expression on Adenomatous Polyposis Coli Mutation-Instigated Tumorigenesis in Mouse Colon and Ovarian Epithelium.
EphrinB2 controls vessel pruning through STAT1-JNK3 signalling.
GluA2 trafficking is involved in apoptosis of retinal ganglion cells induced by activation of EphB/EphrinB reverse signaling in a rat chronic ocular hypertension model.
Biomaterial microenvironments to support the generation of new neurons in the adult brain.
EphrinB2 affects apical constriction in Xenopus embryos and is regulated by ADAM10 and flotillin-1.
EphrinB2 reverse signaling protects against capillary rarefaction and fibrosis after kidney injury.
Neuropsin cleaves EphB2 in the amygdala to control anxiety.
Hedgehog signaling induces arterial endothelial cell formation by repressing venous cell fate.
Soluble EphB4 inhibition of PDGF-induced RPE migration in vitro.
Dynamic changes occur in patterns of endometrial EFNB2/EPHB4 expression during the period of spiral arterial modification in mice.
EphrinB2 regulation by PTH and PTHrP revealed by molecular profiling in differentiating osteoblasts.
The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization.
Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells.
Coucha M, Barrett AC, Elgebaly M, Ergul A, Abdelsaid M
PloS one 2019;14(1):e0210523
PloS one 2019;14(1):e0210523
EPHB6 controls catecholamine biosynthesis by up-regulating tyrosine hydroxylase transcription in adrenal gland chromaffin cells.
Shi W, Wang Y, Peng J, Qi S, Vitale N, Kaneda N, Murata T, Luo H, Wu J
The Journal of biological chemistry 2019 Apr 26;294(17):6871-6887
The Journal of biological chemistry 2019 Apr 26;294(17):6871-6887
Histochemical assessment for osteoblastic activity coupled with dysfunctional osteoclasts in c-src deficient mice.
Toray H, Hasegawa T, Sakagami N, Tsuchiya E, Kudo A, Zhao S, Moritani Y, Abe M, Yoshida T, Yamamoto T, Yamamoto T, Oda K, Udagawa N, Luiz de Freitas PH, Li M
Biomedical research (Tokyo, Japan) 2017;38(2):123-134
Biomedical research (Tokyo, Japan) 2017;38(2):123-134
Embryonic Stem Cell Differentiation to Functional Arterial Endothelial Cells through Sequential Activation of ETV2 and NOTCH1 Signaling by HIF1α.
Tsang KM, Hyun JS, Cheng KT, Vargas M, Mehta D, Ushio-Fukai M, Zou L, Pajcini KV, Rehman J, Malik AB
Stem cell reports 2017 Sep 12;9(3):796-806
Stem cell reports 2017 Sep 12;9(3):796-806
EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance.
Depner C, Zum Buttel H, Böğürcü N, Cuesta AM, Aburto MR, Seidel S, Finkelmeier F, Foss F, Hofmann J, Kaulich K, Barbus S, Segarra M, Reifenberger G, Garvalov BK, Acker T, Acker-Palmer A
Nature communications 2016 Jul 29;7:12329
Nature communications 2016 Jul 29;7:12329
Tissue-Specific Effects of Reduced β-catenin Expression on Adenomatous Polyposis Coli Mutation-Instigated Tumorigenesis in Mouse Colon and Ovarian Epithelium.
Feng Y, Sakamoto N, Wu R, Liu JY, Wiese A, Green ME, Green M, Akyol A, Roy BC, Zhai Y, Cho KR, Fearon ER
PLoS genetics 2015 Nov;11(11):e1005638
PLoS genetics 2015 Nov;11(11):e1005638
EphrinB2 controls vessel pruning through STAT1-JNK3 signalling.
Salvucci O, Ohnuki H, Maric D, Hou X, Li X, Yoon SO, Segarra M, Eberhart CG, Acker-Palmer A, Tosato G
Nature communications 2015 Mar 26;6:6576
Nature communications 2015 Mar 26;6:6576
GluA2 trafficking is involved in apoptosis of retinal ganglion cells induced by activation of EphB/EphrinB reverse signaling in a rat chronic ocular hypertension model.
Dong LD, Gao F, Wang XH, Miao Y, Wang SY, Wu Y, Li F, Wu J, Cheng XL, Sun XH, Yang XL, Wang Z
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Apr 1;35(13):5409-21
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Apr 1;35(13):5409-21
Biomaterial microenvironments to support the generation of new neurons in the adult brain.
Conway A, Schaffer DV
Stem cells (Dayton, Ohio) 2014 May;32(5):1220-9
Stem cells (Dayton, Ohio) 2014 May;32(5):1220-9
EphrinB2 affects apical constriction in Xenopus embryos and is regulated by ADAM10 and flotillin-1.
Ji YJ, Hwang YS, Mood K, Cho HJ, Lee HS, Winterbottom E, Cousin H, Daar IO
Nature communications 2014 Mar 24;5:3516
Nature communications 2014 Mar 24;5:3516
EphrinB2 reverse signaling protects against capillary rarefaction and fibrosis after kidney injury.
Kida Y, Ieronimakis N, Schrimpf C, Reyes M, Duffield JS
Journal of the American Society of Nephrology : JASN 2013 Mar;24(4):559-72
Journal of the American Society of Nephrology : JASN 2013 Mar;24(4):559-72
Neuropsin cleaves EphB2 in the amygdala to control anxiety.
Attwood BK, Bourgognon JM, Patel S, Mucha M, Schiavon E, Skrzypiec AE, Young KW, Shiosaka S, Korostynski M, Piechota M, Przewlocki R, Pawlak R
Nature 2011 May 19;473(7347):372-5
Nature 2011 May 19;473(7347):372-5
Hedgehog signaling induces arterial endothelial cell formation by repressing venous cell fate.
Williams C, Kim SH, Ni TT, Mitchell L, Ro H, Penn JS, Baldwin SH, Solnica-Krezel L, Zhong TP
Developmental biology 2010 May 1;341(1):196-204
Developmental biology 2010 May 1;341(1):196-204
Soluble EphB4 inhibition of PDGF-induced RPE migration in vitro.
He S, Kumar SR, Zhou P, Krasnoperov V, Ryan SJ, Gill PS, Hinton DR
Investigative ophthalmology & visual science 2010 Jan;51(1):543-52
Investigative ophthalmology & visual science 2010 Jan;51(1):543-52
Dynamic changes occur in patterns of endometrial EFNB2/EPHB4 expression during the period of spiral arterial modification in mice.
Zhang J, Dong H, Wang B, Zhu S, Croy BA
Biology of reproduction 2008 Sep;79(3):450-8
Biology of reproduction 2008 Sep;79(3):450-8
EphrinB2 regulation by PTH and PTHrP revealed by molecular profiling in differentiating osteoblasts.
Allan EH, Häusler KD, Wei T, Gooi JH, Quinn JM, Crimeen-Irwin B, Pompolo S, Sims NA, Gillespie MT, Onyia JE, Martin TJ
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2008 Aug;23(8):1170-81
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2008 Aug;23(8):1170-81
The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization.
Yang NY, Pasquale EB, Owen LB, Ethell IM
The Journal of biological chemistry 2006 Oct 27;281(43):32574-86
The Journal of biological chemistry 2006 Oct 27;281(43):32574-86
Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells.
Füller T, Korff T, Kilian A, Dandekar G, Augustin HG
Journal of cell science 2003 Jun 15;116(Pt 12):2461-70
Journal of cell science 2003 Jun 15;116(Pt 12):2461-70
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Ephrin-B2 by Western Blot. Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Ephrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF496) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Ephrin-B2 at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Ephrin-B2 in Rat Hippo-campal Neurons. Ephrin-B2 was detected in immersion fixed rat hippocampal neurons using 2 µg/mL Goat Anti-Human/Mouse/Rat Ephrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF496) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Ephrin-B2 in SH-SY5Y Human Cell Line by Flow Cytometry. SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/Rat Ephrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF496, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
