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LearnPA5-21501
antibody from Invitrogen Antibodies
Targeting: AKR1C4
3-alpha-HSD, C11, CDR, CHDR, DD4, HAKRA, MGC22581
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-21501 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AKR1C4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HepG2. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.83 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using AKR1C4 Polyclonal Antibody (Product # PA5-21501). Sample (30 µg of whole cell lysate). Lane A: Hela. 10% SDS PAGE. HSD3a antibody . AKR1C4 Polyclonal Antibody (Product # PA5-21501) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AKR1C4 Polyclonal Antibody detects HSD3a protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSD3a protein stained by AKR1C4 Polyclonal Antibody (Product # PA5-21501) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human gastric cancer, using AKR1C4 (Product # PA5-21501) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
