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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-61850 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RFX2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VMGEFNDLAS LSLTLLDKDD MGDEQRGSEA GPDARSLGEP LVKRERSDPN HSLQGI Highest antigen sequence identity to the following orthologs: Mouse - 80%, Rat - 80%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.08 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RFX2 in human cell line SCLC-21H using a RFX2 Polyclonal Antibody (Product # PA5-61850).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RFX2 in control (vector only transfected HEK293T lysate) and RFX2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a RFX2 Polyclonal Antibody (Product # PA5-61850).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of RFX2 in human cell line MCF7 using a RFX2 Polyclonal Antibody (Product # PA5-61850) shows localization to nucleoplasm, cytosol and the Golgi apparatus.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RFX2 in human testis tissue shows moderate nuclear positivity in cells of seminiferus ducts. Samples were probed using a RFX2 Polyclonal Antibody (Product # PA5-61850).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RFX2 in human testis and prostate tissues using RFX2 Polyclonal Antibody (Product # PA5-61850). Corresponding RFX2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RFX2 in human prostate using RFX2 Polyclonal Antibody (Product # PA5-61850) shows low expression as expected.
