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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- HPA048969 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA048969, RRID:AB_2680577
- Product name
- Anti-RFX2
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human RFX2, Gene description: regulatory factor X, 2 (influences HLA class II expression), Alternative Gene Names: FLJ14226, Validated applications: WB, IHC, ICC, ChIP, Uniprot ID: P48378, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Primary cilia promote the differentiation of human neurons through the WNT signaling pathway
Loss of RREB1 in pancreatic beta cells reduces cellular insulin content and affects endocrine cell gene expression
MOF-mediated histone H4 Lysine 16 acetylation governs mitochondrial and ciliary functions by controlling gene promoters
Topography of transcriptionally active chromatin in glioblastoma
Mutations in TP73 cause impaired mucociliary clearance and lissencephaly
Interplay of RFX transcription factors 1, 2 and 3 in motile ciliogenesis
Coschiera A, Yoshihara M, Lauter G, Ezer S, Pucci M, Li H, Kavšek A, Riedel C, Kere J, Swoboda P
BMC Biology 2024;22(1)
BMC Biology 2024;22(1)
Loss of RREB1 in pancreatic beta cells reduces cellular insulin content and affects endocrine cell gene expression
Mattis K, Krentz N, Metzendorf C, Abaitua F, Spigelman A, Sun H, Ikle J, Thaman S, Rottner A, Bautista A, Mazzaferro E, Perez-Alcantara M, Manning Fox J, Torres J, Wesolowska-Andersen A, Yu G, Mahajan A, Larsson A, MacDonald P, Davies B, den Hoed M, Gloyn A
Diabetologia 2023;66(4):674-694
Diabetologia 2023;66(4):674-694
MOF-mediated histone H4 Lysine 16 acetylation governs mitochondrial and ciliary functions by controlling gene promoters
Wang D, Li H, Chandel N, Dou Y, Yi R
Nature Communications 2023;14(1)
Nature Communications 2023;14(1)
Topography of transcriptionally active chromatin in glioblastoma
Xu L, Chen Y, Huang Y, Sandanaraj E, Yu J, Lin R, Dakle P, Ke X, Chong Y, Koh L, Mayakonda A, Nacro K, Hill J, Huang M, Gery S, Lim S, Huang Z, Xu Y, Chen J, Bai L, Wang S, Wakimoto H, Yeo T, Ang B, Müschen M, Tang C, Tan T, Koeffler H
Science Advances 2021;7(18)
Science Advances 2021;7(18)
Mutations in TP73 cause impaired mucociliary clearance and lissencephaly
Wallmeier J, Bracht D, Alsaif H, Dougherty G, Olbrich H, Cindric S, Dzietko M, Heyer C, Teig N, Thiels C, Faqeih E, Al-Hashim A, Khan S, Mogarri I, Almannai M, Al Otaibi W, Alkuraya F, Koerner-Rettberg C, Omran H
The American Journal of Human Genetics 2021;108(7):1318-1329
The American Journal of Human Genetics 2021;108(7):1318-1329
Interplay of RFX transcription factors 1, 2 and 3 in motile ciliogenesis
Lemeille S, Paschaki M, Baas D, Morlé L, Duteyrat J, Ait-Lounis A, Barras E, Soulavie F, Jerber J, Thomas J, Zhang Y, Holtzman M, Kistler W, Reith W, Durand B
Nucleic Acids Research 2020;48(16):9019-9036
Nucleic Acids Research 2020;48(16):9019-9036
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Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, cytosol & the Golgi apparatus.
- Sample type
- Human
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human testis and liver tissues using HPA048969 antibody. Corresponding RFX2 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- ChIP-Exo-Seq composite graph for Anti-RFX2 (HPA048969, Lot 000025281) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
