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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-64196 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RIC3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: LSKGKTTAED GKCYTAMPGN THRKITSFEL AQLQEKLKET EAAMEKLINR VGPNGERAQT VTSDQEKRLL HQLREITRVM Highest antigen sequence identity to the following orthologs: Mouse - 88%, Rat - 89%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.05 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Why Does Knocking Out NACHO, But Not RIC3, Completely Block Expression of α7 Nicotinic Receptors in Mouse Brain?
Deshpande A, Vinayakamoorthy RM, Garg BK, Thummapudi JP, Oza G, Adhikari K, Agarwal A, Dalvi P, Iyer S, Thulasi Raman S, Ramesh V, Rameshbabu A, Rezvaya A, Sukumaran S, Swaminathan S, Tilak B, Wang Z, Tran PV, Loring RH
Biomolecules 2020 Mar 19;10(3)
Biomolecules 2020 Mar 19;10(3)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RIC3 in human pancreas using RIC3 Polyclonal Antibody (Product # PA5-64196) shows strong granular cytoplasmic positivity in exocrine glandular cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Assessment of RIC3 antibodies by Western blot. ( A ) Anti-DDK shows RIC3 expression efficiency following cell transfections. DDK-tagged RIC3s showed as single bands around 40 kD with considerable differences among different species. Rat RIC3 was not tagged with DDK. ( B ) Thermo-Fisher anti-hRIC3 PA5-64196 (1:1000) recognizes both splice variants of human and mouse RIC3 (with multiple bands) and weakly stains rat RIC3, but not Xenopus. ( C ) Alomone Laboratories anti-RIC3 antibody (ANC-020, 1:1000) showed a similar but weaker pattern and recognized both splice variants of human and mouse RIC3 (with additional bands) and weakly rat RIC3. A high MW (~100 kD) band is non-specific due to its presence in the control. Numbers in parentheses refer to the amount of protein added to each well to account for differences in protein expression between transfections.
