Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-111849 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RIC3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Applications Tested: This SJ25C1 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RIC3 was performed in human SH-SY5Y neuroblastoma cell line lysate. Lane 1: RIC3 Antibody (Product # PA5-111849) at a dilution of 1:200. Lane 2: RIC3 Antibody preincubated with the negative control antigen.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RIC3 was performed in mouse heart membranes (lanes 1 and 4), rat heart lysate (lanes 2 and 5) and rat brain lysate (lanes 3 and 6). Lane 1-3: RIC3 Antibody (Product # PA5-111849) at a dilution of 1:200. Lane 4-6: RIC3 Antibody preincubated with the negative control antigen.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RIC3 in perfusion-fixed, frozen mouse olfactory bulb and fornix tissue sections using RIC3 Antibody (Product # PA5-111849) at a dilution of 1:400, followed by goat-anti-rabbit-AlexaFluor-488. A) RIC-3 staining in mouse olfactory bulb shows staining in the external plexiform layer (EPL) and in some glomeruli (G). Arrows point at glomeruli with RIC-3 positive core. B) RIC-3 staining in mouse fornix is detected in neuronal profiles (vertical arrows) and neuronal processes (horizontal arrow). Cell nuclei are stained with DAPI (blue).