Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ANC-020-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RIC3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 25 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate: - 1. Anti-RIC3 Antibody (#ANC-020), (1:200). 2. Anti-RIC3 Antibody , preincubated with RIC3 Blocking Peptide (#BLP-NC020).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse heart membranes (lanes 1 and 4), rat heart lysate (lanes 2 and 5) and rat brain lysate (lanes 3 and 6): - 1-3. Anti-RIC3 Antibody (#ANC-020), (1:200).4-6. Anti-RIC3 Antibody , preincubated with RIC3 Blocking Peptide (#BLP-NC020).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of RIC-3 in mouseolfactory bulbandfornix - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-RIC3 Antibody (#ANC-020), (1:400), followed by goat- Anti-rabbit-AlexaFluor-488. A. RIC-3 staining in mouse olfactory bulb shows staining in the external plexiform layer (EPL) and in some glomeruli (G). Arrows point at glomeruli with RIC-3 positive core. B. RIC-3 staining in mouse fornix is detected in neuronal profiles (vertical arrows) and neuronal processes (horizontal arrow). Cell nuclei are stained with DAPI (blue).