HPA009300

CD93 antibody from Atlas Antibodies

C1qR(P), C1QR1, C1qRP, CDw93, dJ737E23.1, ECSM3, MXRA4

Eligible for validation within the Antibodypedia Validation Initiative

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Supportive data in Antibodypedia

Immunohistochemistry

Enhanced validation

Provider product page for HPA009300

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Antibody Data

Product number

HPA009300

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA009300, RRID:AB_1233792

Product name

Anti-CD93

Provider product page

Antibody type

Polyclonal

Description

Affinity purified using the PrEST antigen as affinity ligand

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

FNTQGSFHCGCLPGWVLAPNGVSCTMGPVSLGPPS
GPPDEEDKGEKEGSTVPRAATASPTRGPEGTPKAT
PTTSRPSLSSDAPITSAPLKMLAPSGSSGVWREPS
IHHATAASGPQEPAGGDSSVATQNNDGTDGQKL


Isotype

IgG

Vial size

100 µl

Storage

Store at +4¡C for short term storage. Long time storage is recommended at -20¡C.

References

Transcriptional profiling of human glioblastoma vessels indicates a key role of VEGF-A and TGFβ2 in vascular abnormalization
Dieterich L, Mellberg S, Langenkamp E, Zhang L, Zieba A, Salomäki H, Teichert M, Huang H, Edqvist P, Kraus T, Augustin H, Olofsson T, Larsson E, Söderberg O, Molema G, Pontén F, Georgii-Hemming P, Alafuzoff I, Dimberg A
The Journal of Pathology 2012 November;228(3):378-390

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Negative control (vector only transfected HEK293T lysate)Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415997)

Sample type

HUMAN

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to plasma membrane & vesicles.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in megakaryocytes.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human gallbladder shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows low expression as expected.

Sample type

HUMAN

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human gallbladder and pancreas tissues using Anti-CD93 antibody. Corresponding CD93 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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