PA5-101343

antibody from Invitrogen Antibodies

Targeting: AGPAT3 LPAAT-gamma, LPAAT3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-101343

Provider

Invitrogen Antibodies

Product name

AGPAT3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Antibody detects endogenous levels of total AGPAT3.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

Ferroptosis response segregates small cell lung cancer (SCLC) neuroendocrine subtypes.

Bebber CM, Thomas ES, Stroh J, Chen Z, Androulidaki A, Schmitt A, Höhne MN, Stüker L, de Pádua Alves C, Khonsari A, Dammert MA, Parmaksiz F, Tumbrink HL, Beleggia F, Sos ML, Riemer J, George J, Brodesser S, Thomas RK, Reinhardt HC, von Karstedt S
Nature communications 2021 Apr 6;12(1):2048

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AGPAT3 in mouse cancer (Lane 1: treated with blocking peptide). Samples were incubated with AGPAT3 polyclonal antibody (Product # PA5-101343).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of AGPAT3 in K562. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with AGPAT3 polyclonal antibody (Product # PA5-101343) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4 non-NE/NE SCLC subtypes undergo lipid metabolism remodeling. a Heatmap showing the representation of mono-oxidized phospholipid species (PE phosphatidylethanolamine; PC phosphatidylcholine) in 181.5 stickers as compared to 181.5 floaters treated with either DMSO or RSL3 [1 uM] for 5 h and then subjected to lipidomics. Samples for each condition ( n = 5) were averaged and normalized to the cell number (2.5 x 10 6 ). Each lipid species was normalized to levels detected in floaters +DMSO. One representative out of two independent experiments is shown. Heatmap color code indicates normalized lipid species levels of each sample. b - e 181.5 stickers floaters ( n = 5 samples) as compared to 181.5 floaters ( n = 5 samples) were analyzed for basal diacylglycerol (DAG) and ether-linked lipids by mass spectrometry. Lipid content was normalized to infused protein for each condition and replicate. Individual PUFAs (4 double bonds or more) are plotted. f RNA was isolated from three stickers / floaters lines (RP181.5; RP246.7; BYC5.1), respective cDNA obtained and qPCR performed for the indicated transcripts. g RP181.5 were subjected to the indicated siRNA-mediated knockdowns for 72 h and then treated with RSL3 [1 uM] for an additional 24 h. DRAQ7 [0.1 uM] was added to all wells to visualize dead cells. Images were acquired every 2 h using the IncuCyte S3 bioimaging platform. Dead cells/image are normalized to cell confluence at the beginning of RSL3 treatment. h Representative west