44-426G

antibody from Invitrogen Antibodies

Targeting: JAK2 JTK10

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

44-426G

Provider

Invitrogen Antibodies

Product name

Phospho-JAK2 (Tyr1007, Tyr1008) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20°C

References

Submitted references

Krüppel-like factor 7 attenuates hippocampal neuronal injury after traumatic brain injury.

Li WY, Fu XM, Wang ZD, Li ZG, Ma D, Sun P, Liu GB, Zhu XF, Wang Y
Neural regeneration research 2022 Mar;17(3):661-672

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Chronic exposure to methylmercury enhances the anorexigenic effects of leptin in C57BL/6J male mice.

Ferrer B, Prince LM, Tinkov AA, Santamaria A, Farina M, Rocha JB, Bowman AB, Aschner M
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2021 Jan;147:111924

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JAK2 tyrosine kinase phosphorylates and is negatively regulated by centrosomal protein Ninein.

Jay J, Hammer A, Nestor-Kalinoski A, Diakonova M
Molecular and cellular biology 2015 Jan;35(1):111-31

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Cysteine induces longitudinal bone growth in mice by upregulating IGF-I.

Moon PD, Kim MH, Oh HA, Nam SY, Han NR, Jeong HJ, Kim HM
International journal of molecular medicine 2015 Aug;36(2):571-6

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Urokinase-type plasminogen activator receptor signaling is critical in nasopharyngeal carcinoma cell growth and metastasis.

Bao YN, Cao X, Luo DH, Sun R, Peng LX, Wang L, Yan YP, Zheng LS, Xie P, Cao Y, Liang YY, Zheng FJ, Huang BJ, Xiang YQ, Lv X, Chen QY, Chen MY, Huang PY, Guo L, Mai HQ, Guo X, Zeng YX, Qian CN
Cell cycle (Georgetown, Tex.) 2014;13(12):1958-69

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Pharmacologic suppression of JAK1/2 by JAK1/2 inhibitor AZD1480 potently inhibits IL-6-induced experimental prostate cancer metastases formation.

Gu L, Talati P, Vogiatzi P, Romero-Weaver AL, Abdulghani J, Liao Z, Leiby B, Hoang DT, Mirtti T, Alanen K, Zinda M, Huszar D, Nevalainen MT
Molecular cancer therapeutics 2014 May;13(5):1246-58

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Molecular basis for pseudokinase-dependent autoinhibition of JAK2 tyrosine kinase.

Shan Y, Gnanasambandan K, Ungureanu D, Kim ET, Hammarén H, Yamashita K, Silvennoinen O, Shaw DE, Hubbard SR
Nature structural & molecular biology 2014 Jul;21(7):579-84

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IL-21 and CD40L synergistically promote plasma cell differentiation through upregulation of Blimp-1 in human B cells.

Ding BB, Bi E, Chen H, Yu JJ, Ye BH
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 15;190(4):1827-36

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IL-6 promotes head and neck tumor metastasis by inducing epithelial-mesenchymal transition via the JAK-STAT3-SNAIL signaling pathway.

Yadav A, Kumar B, Datta J, Teknos TN, Kumar P
Molecular cancer research : MCR 2011 Dec;9(12):1658-67

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Prolactin can modulate CD4+ T-cell response through receptor-mediated alterations in the expression of T-bet.

Tomio A, Schust DJ, Kawana K, Yasugi T, Kawana Y, Mahalingaiah S, Fujii T, Taketani Y
Immunology and cell biology 2008 Oct;86(7):616-21

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Erythropoietin prevents dialysis fluid-induced apoptosis of mesothelial cells.

Vorobiov M, Malki M, Shnaider A, Basok A, Rogachev B, Lewis EC, Chaimovitz C, Zlotnik M, Douvdevani A
Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis 2008 Nov-Dec;28(6):648-54

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Molecular mechanisms involved in interleukin-4-induced human neutrophils: expression and regulation of suppressor of cytokine signaling.

Ratthé C, Pelletier M, Chiasson S, Girard D
Journal of leukocyte biology 2007 May;81(5):1287-96

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Dampening of IFN-gamma-inducible gene expression in human choriocarcinoma cells is due to phosphatase-mediated inhibition of the JAK/STAT-1 pathway.

Choi JC, Holtz R, Petroff MG, Alfaidy N, Murphy SP
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 1;178(3):1598-607

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Erythropoietin induces sustained phosphorylation of STAT5 in primitive but not definitive erythrocytes generated from mouse embryonic stem cells.

Tsuji-Takayama K, Otani T, Inoue T, Nakamura S, Motoda R, Kibata M, Orita K
Experimental hematology 2006 Oct;34(10):1323-32

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Distinct and gradient distributions of connexin26 and connexin30 in the cochlear sensory epithelium of guinea pigs.

Zhao HB, Yu N
The Journal of comparative neurology 2006 Nov 20;499(3):506-18

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G protein coupling and second messenger generation are indispensable for metalloprotease-dependent, heparin-binding epidermal growth factor shedding through angiotensin II type-1 receptor.

Mifune M, Ohtsu H, Suzuki H, Nakashima H, Brailoiu E, Dun NJ, Frank GD, Inagami T, Higashiyama S, Thomas WG, Eckhart AD, Dempsey PJ, Eguchi S
The Journal of biological chemistry 2005 Jul 15;280(28):26592-9

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Falcarindiol impairs the expression of inducible nitric oxide synthase by abrogating the activation of IKK and JAK in rat primary astrocytes.

Shiao YJ, Lin YL, Sun YH, Chi CW, Chen CF, Wang CN
British journal of pharmacology 2005 Jan;144(1):42-51

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Role of tyrosine 441 of interferon-gamma receptor subunit 1 in SOCS-1-mediated attenuation of STAT1 activation.

Qing Y, Costa-Pereira AP, Watling D, Stark GR
The Journal of biological chemistry 2005 Jan 21;280(3):1849-53

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Mechanism of protein tyrosine phosphatase 1B-mediated inhibition of leptin signalling.

Lund IK, Hansen JA, Andersen HS, Møller NP, Billestrup N
Journal of molecular endocrinology 2005 Apr;34(2):339-51

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Activation of the JAK/STAT pathway in vascular smooth muscle by serotonin.

Banes AK, Shaw SM, Tawfik A, Patel BP, Ogbi S, Fulton D, Marrero MB
American journal of physiology. Cell physiology 2005 Apr;288(4):C805-12

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Growth arrest of epithelial cells during measles virus infection is caused by upregulation of interferon regulatory factor 1.

Yokota S, Okabayashi T, Yokosawa N, Fujii N
Journal of virology 2004 May;78(9):4591-8

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Growth arrest of epithelial cells during measles virus infection is caused by upregulation of interferon regulatory factor 1.

Yokota S, Okabayashi T, Yokosawa N, Fujii N
Journal of virology 2004 May;78(9):4591-8

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Protein tyrosine phosphatase 1B negatively regulates leptin signaling in a hypothalamic cell line.

Kaszubska W, Falls HD, Schaefer VG, Haasch D, Frost L, Hessler P, Kroeger PE, White DW, Jirousek MR, Trevillyan JM
Molecular and cellular endocrinology 2002 Sep 30;195(1-2):109-18

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Protein tyrosine phosphatase 1B negatively regulates leptin signaling in a hypothalamic cell line.

Kaszubska W, Falls HD, Schaefer VG, Haasch D, Frost L, Hessler P, Kroeger PE, White DW, Jirousek MR, Trevillyan JM
Molecular and cellular endocrinology 2002 Sep 30;195(1-2):109-18

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C-terminal region of STAT-1alpha is not necessary for its ubiquitination and degradation caused by mumps virus V protein.

Yokosawa N, Yokota S, Kubota T, Fujii N
Journal of virology 2002 Dec;76(24):12683-90

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C-terminal region of STAT-1alpha is not necessary for its ubiquitination and degradation caused by mumps virus V protein.

Yokosawa N, Yokota S, Kubota T, Fujii N
Journal of virology 2002 Dec;76(24):12683-90

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Reactive oxygen species regulate heat-shock protein 70 via the JAK/STAT pathway.

Madamanchi NR, Li S, Patterson C, Runge MS
Arteriosclerosis, thrombosis, and vascular biology 2001 Mar;21(3):321-6

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Reactive oxygen species regulate heat-shock protein 70 via the JAK/STAT pathway.

Madamanchi NR, Li S, Patterson C, Runge MS
Arteriosclerosis, thrombosis, and vascular biology 2001 Mar;21(3):321-6

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Hyperglycemia enhances angiotensin II-induced janus-activated kinase/STAT signaling in vascular smooth muscle cells.

Amiri F, Venema VJ, Wang X, Ju H, Venema RC, Marrero MB
The Journal of biological chemistry 1999 Nov 5;274(45):32382-6

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Hyperglycemia enhances angiotensin II-induced janus-activated kinase/STAT signaling in vascular smooth muscle cells.

Amiri F, Venema VJ, Wang X, Ju H, Venema RC, Marrero MB
The Journal of biological chemistry 1999 Nov 5;274(45):32382-6

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Phospho-JAK2 (Tyr1007, Tyr1008) (Product # 44-426G) and ~130 kDa band corresponding to JAK2 (Tyr1007, Tyr1008) was observed. Whole cell extracts of 3T3-L1 lysate (Lane 1), 3T3-L1 treated with 50 ng/mL of Leukemia Inhibitory Factor (LIF) for 15 min (lane 2), Antibody treated with non-phospho peptide (Lane 3) and Antibody treated with phospho peptide (Lane 4) were electrophoresed using Novex® NuPAGE® Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1:1000 dilution) and detected with Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Involvement of the JAK2/STAT3 signaling pathway in the neuroprotective effects of KLF7. (A) Representative western blot of p-JAK2, t-JAK2, p-STAT3, and t-STAT3 expression in cultured HT22 cells at 1 day after stretch and OGD treatment. (B, C) Quantitative results of the relative optical densities of p-JAK2/t-JAK2 and p-STAT3/t-STAT3. (D) Co-immunoprecipitation analysis of the interactions between KLF7 and p-STAT3 in stretch- and OGD-damaged HT22 cells at 1 day, revealing a physical interaction between KLF7 and p-STAT3. Data are expressed as the mean +- SD. Each experiment was repeated three times. * P < 0.05, vs . control group; # P < 0.05, vs . stretch + OGD + AAV-NC group; & P < 0.05, vs . stretch + OGD + AAV-KLF7 group (one-way analysis of variance followed by Tukey's post hoc test). AAV: Adeno-associated virus; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; IB: immunoblotting; IP: immunoprecipitation; KLF7: Kruppel-like factor 7; NC: negative control; OGD: oxygen-glucose deprivation; p-JAK2: phospho-Janus kinase 2; t-JAK2: total-Janus kinase 2; p-STAT3: phospho-signal transducer and activator of transcription 3; t-STAT3: total-signal transducer and activator of transcription 3.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 9 KLF7 activates the JAK2/STAT3 pathway at 3 days after TBI. (A) Representative western blots of p-JAK2, t-JAK2, p-STAT3, and t-STAT3 expression in ipsilateral hippocampal tissue. (B, C) Quantitative results of the p-JAK2/t-JAK2 and p-STAT3/t-STAT3 ratios. Data are expressed as the mean +- SD ( n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Tukey's post hoc test). AAV: Adeno-associated virus; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; KLF7: Kruppel-like factor 7; NC: negative control; p-JAK2: phospho-Janus kinase 22; p-STAT3: phospho-signal transducer and activator of transcription 3; t-JAK2: total-Janus kinase; t-STAT3: total-signal transducer and activator of transcription 3; TBI: traumatic brain injury.