710928

antibody from Invitrogen Antibodies

Targeting: JAK2 JTK10

Provider product page for 710928

Western blot

Immunocytochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [2]
Comments [0]
Validations
Immunocytochemistry [2]
Other assay [1]

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Product number: 710928 - Provider product page
Provider: Invitrogen Antibodies
Product name: Phospho-JAK2 (Tyr1007, Tyr1008) Recombinant Superclonal™ Antibody (18HCLC)
Antibody type: Other
Antigen: Synthetic peptide
Description: This antibody is predicted to react with Monkey, Pig, Sheep and Bovine. Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Antibody clone number: 18HCLC
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

JAK2 protein structure - 710928 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: For immunofluorescence analysis HT-29 cells were fixed and permeabilized for detection of Jak (pY1007/1008) using Jak (pY1007/1008) Recombinant Rabbit Polyclonal Antibody (Product # 710928, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938), and Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Detection and localization of Jak (pY1007/1008) (green) in the cytoplasmic can be clearly observed in cells treated with MG132 (20 uM, 1 hr) and IFN-gamma (100 ng, 15 min) as compared to untreated cells. Antibody specificity was demonstrated by competition with the Jak (pY1007/1008) peptide, which results in loss of signal. No competition was observed with the non-phospho peptide.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: For immunofluorescence analysis HT-29 cells were fixed and permeabilized for detection of Jak (pY1007/1008) using Jak (pY1007/1008) Recombinant Rabbit Superclonal™ Antibody (Product # 710928, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938), and Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Detection and localization of Jak (pY1007/1008) (green) in the cytoplasmic can be clearly observed in cells treated with MG132 (20 uM, 1 hr) and IFN-gamma (100 ng, 15 min) as compared to untreated cells. Antibody specificity was demonstrated by competition with the Jak (pY1007/1008) peptide, which results in loss of signal. No competition was observed with the non-phospho peptide.