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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- RQ4762 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- JAK2 Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific JAK2 antibody is suitable for use in Immunofluorescence/Immunocytochemistry/Immunohistochemistry/Western blot applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- COG-10
- Vial size
- 100 ul
- Concentration
- Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA
- Storage
- Store the JAK2 antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human Jurkat cell lysate with JAK2 antibody. Predicted molecular weight ~130 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HepG2, 2) human ThP-1, 3) rat NRK, 4) rat PC-12 and 5) mouse RAW264.7 cell lysate with JAK2 antibody. Predicted molecular weight ~130 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC staining of FFPE human Jurkat cells with JAK2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human A549 cells with JAK2 antibody (green) and Beta Tubulin antibody (red). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human prostate cancer tissue with JAK2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
