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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX101844 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101844, RRID:AB_1241286
- Product name
- Ribosome binding protein 1 antibody [C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Ribosome binding protein 1 antibody validation by siRNA knock-down.Upperpanel: Ribosome binding protein 1 antibody GTX101844Lower panel: Vinculin antibody (GTX109749)A. 30 ?g HepG2 whole cell lysate/extract B. 30 ?g whole cell lysate/extract of RRBP1 siRNA#1-transfected HepG2 cells C. 30 ?g whole cell lysate/extract of RRBP1 siRNA#2-transfected HepG2 cells5% SDS-PAGERibosome binding protein 1 antibody (GTX101844) dilution: 1:500Vinculin antibody (GTX109749) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate)A: Hep G2 (GTX27900)7.5% SDS PAGEGTX101844 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A:NIH-3T35% SDS PAGE GTX101844 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Ribosome binding protein 1(GTX101844) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Ribosome binding protein 1(GTX101844) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using Ribosome binding protein 1(GTX101844) antibody at 1:500 dilution.
