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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- NBP1-32813 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-32813, RRID:AB_2180227
- Product name
- Rabbit Polyclonal RRBP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RRBP1 Antibody [NBP1-32813] - Sample (30 ug of whole cell lysate) A:NIH-3T3 5% SDS PAGE diluted at 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RRBP1 Antibody [NBP1-32813] - Sample (30 ug of whole cell lysate)A: Hep G2 7. 5% SDS PAGE, antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RRBP1 Antibody [NBP1-32813] - Ribosome binding protein 1 antibody validation by siRNA knock-down. Upper panel: Ribosome binding protein 1 antibody; Lower panel: Vinculin antibody A. 30 ug HepG2 whole cell lysate/extract B. 30 ug whole cell lysate/extract of RRBP1 siRNA#1-transfected HepG2 cells C. 30 ug whole cell lysate/extract of RRBP1 siRNA#2-transfected HepG2 cells 5% SDS-PAGE. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RRBP1 Antibody [NBP1-32813] - OVCAR3 xenograft, using Ribosome binding protein 1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
