17275-1-AP

antibody from Invitrogen Antibodies

Targeting: CALML3 CLP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

17275-1-AP

Provider

Invitrogen Antibodies

Product name

CALML3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MADQLTEEQ VTEFKEAFSL FDKDGDGCIT TRELGTVMRS LGQNPTEAEL RDMMSEIDRD GNGTVDFPEF LGMMARKMKD TDNEEEIREA FRVFDKDGNG FVSAAELRHV MTRLGEKLSD EEVDEMIRAA DTDGDGQVNY EEFVRVLVSK (1-149 aa encoded by BC031889)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.53 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A431 cells were subjected to SDS PAGE followed by western blot with 17275-1-AP ( CALML3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using (Product # 17275-1-AP) (CALML3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of A431 cells using 17275-1-AP (CALML3 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IGG.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 17275-1-AP (CALML3 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 17275-1-AP (CALML3 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-CALML3 (IP:17275-1-AP, 4ug; Detection:17275-1-AP 1:300) with A431 cells lysate 2400 ug.