Antibody data
- Antibody Data
 - Antigen structure
 - References [0]
 - Comments [0]
 - Validations
 - Western blot [2]
 - Immunohistochemistry [2]
 
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Validation data
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- Product number
 - STJ95468 - Provider product page

 - Provider
 - St John's Laboratory
 - Product name
 - Anti-RPL27A antibody (70-150 C-Term) (STJ95468)
 - Antibody type
 - Polyclonal
 - Description
 - Rabbit polyclonal antibody anti-60s Ribosomal Protein L27a (70-150 C-Term) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry and ELISA research applications.
 - Reactivity
 - Human, Mouse, Rat
 - Host
 - Rabbit
 - Conjugate
 - Unconjugated
 - Antigen sequence
 NA- Epitope
 - NA
 - Isotype
 - IgG
 - Antibody clone number
 - NA
 - Vial size
 - NA
 - Concentration
 - NA
 - Storage
 - Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
 - Handling
 - NA
 
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					Supportive validation
					
									
				
Supportive validation
		
				- Submitted by
 - St John's Laboratory (provider)
 - Main image
 
- Experimental details
 - Western blot analysis of lysates from COLO cells, using RPL27A Antibody. The lane on the right is blocked with the synthesized peptide.
 - Sample type
 - NA
 - Validation comment
 - NA
 - Primary Ab dilution
 - NA
 - Other comments
 - NA
 - Secondary Ab
 - NA
 - Secondary Ab dilution
 - NA
 - Protocol
 - NA
 
Supportive validation
- Submitted by
 - St John's Laboratory (provider)
 - Main image
 
- Experimental details
 - Western blot analysis of various cells using Ribosomal Protein L27A Polyclonal Antibody diluted at 1ï¼2000
 - Sample type
 - NA
 - Validation comment
 - NA
 - Primary Ab dilution
 - NA
 - Other comments
 - NA
 - Secondary Ab
 - NA
 - Secondary Ab dilution
 - NA
 - Protocol
 - NA
 
							
					Supportive validation
					
									
				
Supportive validation
		
		- Submitted by
 - St John's Laboratory (provider)
 - Main image
 
- Experimental details
 - Immunofluorescence analysis of A549 cells, using RPL27A Antibody. The picture on the right is blocked with the synthesized peptide.
 - Sample type
 - NA
 - Validation comment
 - NA
 - Primary Ab dilution
 - NA
 - Other comments
 - NA
 - Secondary Ab
 - NA
 - Secondary Ab dilution
 - NA
 - Protocol
 - NA
 
Supportive validation
- Submitted by
 - St John's Laboratory (provider)
 - Main image
 
- Experimental details
 - Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
 - Sample type
 - NA
 - Validation comment
 - NA
 - Primary Ab dilution
 - NA
 - Other comments
 - NA
 - Secondary Ab
 - NA
 - Secondary Ab dilution
 - NA
 - Protocol
 - NA